First molecular detection of Hemolivia and Hepatozoon parasites in reptile-associated ticks on Iriomote Island, Japan

Qiu, Yongjin; Kidera, Noriko; Thu, May June; Hayashi, Masaki; Fujishima, Kanta; Tamura, Hisao

First molecular detection of Hemolivia and Hepatozoon parasites in reptile-associated ticks on Iriomote Island, Japan

Qiu, Yongjin; Kidera, Noriko; Thu, May June et al.

Parasitology research, 2021, 120, 4067-4072

Permalink : https://hdl.handle.net/2115/87062

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アクセス権	open access
URI	https://hdl.handle.net/2115/87062
タイトル	First molecular detection of Hemolivia and Hepatozoon parasites in reptile-associated ticks on Iriomote Island, Japan
著者	Qiu, Yongjin ; Kidera, Noriko ; Thu, May June ; Hayashi, Masaki ; Fujishima, Kanta ; Tamura, Hisao
e-Rad_Researcher 00760985 著者名 Qiu, Yongjin
著者名 Kidera, Noriko
著者名 Thu, May June
著者名 Hayashi, Masaki
著者名 Fujishima, Kanta
著者名 Tamura, Hisao
言語	英語
キーワード	Hemolivia
	Hepatozoon
	Amblyomma geoemydae
	Amblyomma nitidum
	Reptile-associated ticks
	Japan
発行日	2021-11-02
出版者	Springer
収録物名	Parasitology research
巻(号)	120
ページ	4067-4072
抄録	Hepatozoon and Hemolivia are members of the haemogregarines and are reported in reptiles and reptile-associated ticks. However, no studies have reported on Hepatozoon and Hemolivia in Japanese reptile-associated ticks. This study aimed to molecularly identify and to characterize Hepatozoon and Hemolivia in Japanese reptile-associated ticks, Amblyomma geoemydae (Cantor, 1847) and Amblyomma nitidum (Hirst & Hirst, 1910). A total of 41 and 75 DNA samples from A. geoemydae and A. nitidum ticks, respectively, were used for screening of Hepatozoon and Hemolivia with polymerase chain reaction targeting 18S rDNA. As a result, Hemolivia and Hepatozoon were detected in two A. geoemydae and one A. nitidum, respectively. The sequences of Hemolivia spp. showed a 99.5% (1,050/1,055 bp) identity with Hemolivia parvula (KR069083), and the Hemolivia spp. were located in the same clade as H. parvula in the phylogenetic tree. The sequences of Hepatozoon sp. showed a 98.4% (1,521/1,545 bp) identity with Hepatozoon colubri (MN723844), and the Hepatozoon sp. was distinct from validated Hepatozoon species in the tree. Our findings highlight the first molecular record of Hemolivia in Japan and present the first detection of Hepatozoon in A. nitidum. Further investigations on these tick-borne protozoa are required to understand their life cycle and pathogenicity.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a post-peer-review, pre-copyedit version of an article published in Parasitology research. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00436-021-07345-y.
PISSN	0932-0113
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00436-021-07345-y