Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells

Sasaki, Michihito; Kishimoto, Mai; Itakura, Yukari; Tabata, Koshiro; Intaruck, Kittiya; Uemura, Kentaro; Toba, Shinsuke; Sanaki, Takao; Sato, Akihiko; Hall, William W.; Orba, Yasuko; Sawa, Hirofumi

Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells

Sasaki, Michihito; Kishimoto, Mai; Itakura, Yukari et al.

Biochemical and biophysical research communications, 2021, 577, 146-151

Permalink : https://hdl.handle.net/2115/87072

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アクセス権	open access
URI	https://hdl.handle.net/2115/87072
タイトル	Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells
著者	Sasaki, Michihito ; Kishimoto, Mai ; Itakura, Yukari ; Tabata, Koshiro ; Intaruck, Kittiya ; Uemura, Kentaro ; Toba, Shinsuke ; Sanaki, Takao ; Sato, Akihiko ; Hall, William W. ; Orba, Yasuko ; Sawa, Hirofumi
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
著者名 Kishimoto, Mai
著者名 Itakura, Yukari
著者名 Tabata, Koshiro
著者名 Intaruck, Kittiya
著者名 Uemura, Kentaro
著者名 Toba, Shinsuke
著者名 Sanaki, Takao
著者名 Sato, Akihiko
著者名 Hall, William W.
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
言語	英語
キーワード	SARS-CoV-2
	A549 cells
	Air-liquid interface
	ACE2
	TMPRSS2
	Viral entry
発行日	2021-11
出版者	Elsevier
収録物名	Biochemical and biophysical research communications
巻(号)	577
ページ	146-151
抄録	The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARSCoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2. (c) 2021 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0006-291X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2021.09.015