The detectability and removal efficiency of SARS-CoV-2 in a large-scale septic tank of a COVID-19 quarantine facility in Japan

Iwamoto, Ryo; Yamaguchi, Kiyoshi; Arakawa, Chisato et al.

Science of the total environment, 2022, 849, 157869

Permalink : https://hdl.handle.net/2115/87081

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https://doi.org/10.1016/j.scitotenv.2022.157869      

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アクセス権
metadata only access
URI
https://hdl.handle.net/2115/87081
タイトル
The detectability and removal efficiency of SARS-CoV-2 in a large-scale septic tank of a COVID-19 quarantine facility in Japan
著者
Iwamoto, Ryo ; Yamaguchi, Kiyoshi ; Arakawa, Chisato ; Ando, Hiroki ; Haramoto, Eiji ; Setsukinai, Ken-ichi ; Katayama, Kotoe ; Yamagishi, Takuya ; Sorano, Sumire ; Murakami, Michio ; Kyuwa, Shigeru ; Kobayashi, Hiroyuki ; Okabe, Satoshi ; Imoto, Seiya ; Kitajima, Masaaki
著者名
Iwamoto, Ryo
著者名
Yamaguchi, Kiyoshi
著者名
Arakawa, Chisato
著者名
Ando, Hiroki
著者名
Haramoto, Eiji
著者名
Setsukinai, Ken-ichi
著者名
Katayama, Kotoe
著者名
Yamagishi, Takuya
著者名
Sorano, Sumire
著者名
Murakami, Michio
著者名
Kyuwa, Shigeru
e-Rad_Researcher
70374478
著者名
Kobayashi, Hiroyuki
e-Rad_Researcher
10253816
著者名
Okabe, Satoshi
著者名
Imoto, Seiya
著者名
Kitajima, Masaaki
言語
英語
キーワード
SARS-CoV-2
Wastewater-based epidemiology
Wastewater treatment
Activated sludge
Quantification method
発行日
2022-11-25
出版者
Elsevier
収録物名
Science of the total environment
巻(号)
849
ページ
157869
抄録
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to be present in sewage, and wastewater -based epidemiology has attracted much attention. However, the physical partitioning of SARS-CoV-2 in wastewater and the removal efficiency of treatment systems require further investigation. This study aimed to investigate the de-tectability and physical partitioning of SARS-CoV-2 in wastewater and assess its removal in a large-scale septic tank employing anaerobic, anoxic, and oxic processes in a sequential batch reactor, which was installed in a coronavirus disease 2019 (COVID-19) quarantine facility. The amount of SARS-CoV-2 RNA in wastewater was determined with polyethylene glycol (PEG) precipitation followed by quantitative polymerase chain reaction (qPCR), and the association of SARS-CoV-2 with wastewater solids was evaluated by the effect of filtration prior to PEG precipitation (pre-filtration). The amount of SARS-CoV-2 RNA detected from pre-filtered samples was substantially lower than that of samples without pre-filtration. These results suggest that most SARS-CoV-2 particles in wastewater are associ-ated with the suspended solids excluded by pre-filtration. The removal efficiency of SARS-CoV-2 in the septic tank was evaluated based on the SARS-CoV-2 RNA concentrations in untreated and treated wastewater, which was determined by the detection method optimized in this study. Escherichia coli and pepper mild mottle virus (PMMoV) were also quantified to validate the wastewater treatment system's performance. The mean log10 reduction values of SARS-CoV-2, E. coli, and PMMoV were 2.47 (range, 2.25-2.68), 2.81 (range, 2.45-3.18), and 0.66 (range, 0.61-0.70), respec-tively, demonstrating that SARS-CoV-2 removal by the wastewater treatment system was comparable to or better than the removal of fecal indicators. These results suggest that SARS-CoV-2 can be readily removed by the septic tank. This is the first study to determine the removal efficiency of SARS-CoV-2 in a facility-level sequencing batch activated sludge system.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0048-9697
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1016/j.scitotenv.2022.157869