metadata only access

https://hdl.handle.net/2115/87087

The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary

英語

2022-10-28

Oxford University Press

Nucleic acids research

50(19)

10914-10928

Centromeres of most eukaryotes consist of two distinct chromatin domains: a kinetochore domain, identified by the histone H3 variant, CENP-A, and a heterochromatic domain. How these two domains are separated is unclear. Here, we show that, in Schizosaccharomyces pombe, mutation of the chromatin remodeler RSC induced CENP-A(Cnp1) misloading at pericentromeric heterochromatin, resulting in the mis-assembly of kinetochore proteins and a defect in chromosome segregation. We find that RSC functions at the kinetochore boundary to prevent CENP-A(Cnp1) from spreading into neighbouring heterochromatin, where deacetylated histones provide an ideal environment for the spread of CENP-A(Cnp1). In addition, we show that RSC decompacts the chromatin structure at this boundary, and propose that this RSC-directed chromatin decompaction prevents mis-propagation of CENP-A(Cnp1) into pericentromeric heterochromatin. Our study provides an insight into how the distribution of distinct chromatin domains is established and maintained.

学術雑誌論文

Version of Record

0305-1048