In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering

Santhakumar, Syama; Oyane, Ayako; Nakamura, Maki; Koga, Kenji; Miyata, Saori; Muratsubaki, Ko; Miyaji, Hirofumi

In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering

Santhakumar, Syama; Oyane, Ayako; Nakamura, Maki et al.

Materials Science and Engineering: C, 2020, 116, 111194

Permalink : https://hdl.handle.net/2115/87263

このアイテムのアクセス数:384件（2026-01-17 00:29 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/87263
タイトル	In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering
著者	Santhakumar, Syama ; Oyane, Ayako ; Nakamura, Maki ; Koga, Kenji ; Miyata, Saori ; Muratsubaki, Ko ; Miyaji, Hirofumi
著者名 Santhakumar, Syama
著者名 Oyane, Ayako
著者名 Nakamura, Maki
著者名 Koga, Kenji
著者名 Miyata, Saori
著者名 Muratsubaki, Ko
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
言語	英語
キーワード	Amorphous calcium phosphate (ACP)
	Nanoparticles
	Scaffold
	Bone tissue engineering
	Basic fibroblast growth factor (bFGF)
	Nucleation
発行日	2020-11
出版者	Elsevier
収録物名	Materials Science and Engineering: C
巻(号)	116
ページ	111194
抄録	Amorphous calcium phosphate (ACP) plays an important role in biomineralization within the three-dimensional (3D) collagen network in human hard tissues, and exhibits osteoconductivity. Porous collagen sponges coated with ACP nanoparticles could be considered as potential scaffolds for use in bone tissue engineering. In this study, such composite materials were fabricated via homogeneous ACP precipitation using a supersaturated calcium phosphate (CaP) solution. Homogeneous ACP precipitation was induced in situ within the sponges by a temperature-controlled coating process composed of two steps. In the first step, the CaP solution was cooled to 4 °C to suppress precipitation until the solution penetrated fully into the sponge's internal pores. In the second step, the CaP solution was warmed up to 25 °C with continuous shaking to induce ACP precipitation within the sponges. The resulting sponges were therefore coated with ACP nanoparticles on their inner and outer surfaces. A simulated body fluid (SBF) test indicated osteoconductivity of the collagen sponges coated with ACP nanoparticles. Further, ACP-coated collagen sponges immobilizing basic fibroblast growth factor (bFGF) were fabricated using the CaP solution supplemented with bFGF. The fabricated sponges allowed the sustained release of bFGF in a culture medium and enhanced proliferation of osteoblastic MC3T3-E1 cells. Such ACP-coated collagen sponges have the potential to be used as scaffolds in bone tissue engineering if pursued for further in vitro and in vivo studies.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0928-4931
関連情報	PMID 32806285
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.msec.2020.111194