Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture

Saito, Shun; Akizawa, Hiroki; Furukawa, Eri; Yanagawa, Yojiro; Bai, Hanako; Takahashi, Masashi; Kawahara, Manabu

Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture

Saito, Shun; Akizawa, Hiroki; Furukawa, Eri et al.

Journal of reproduction and development, 2022, 68(5), 330-334

Permalink : https://hdl.handle.net/2115/87412

このアイテムのアクセス数:282件（2026-01-13 12:23 集計）

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https://doi.org/10.1262/jrd.2022-054			1

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/87412
タイトル	Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture
著者	Saito, Shun ; Akizawa, Hiroki ; Furukawa, Eri ; Yanagawa, Yojiro ; Bai, Hanako ; Takahashi, Masashi ; Kawahara, Manabu
著者名 Saito, Shun
著者名 Akizawa, Hiroki
著者名 Furukawa, Eri
e-Rad_Researcher 20609656 著者名 Yanagawa, Yojiro
e-Rad_Researcher 60775443 著者名 Bai, Hanako
e-Rad_Researcher 10343964 著者名 Takahashi, Masashi
e-Rad_Researcher 70468700 著者名 Kawahara, Manabu
言語	英語
キーワード	Blastcyst
	Embryo transfer
	Full-term development
	On-gel culture
発行日	2022-10
出版者	Society for Reproduction and Development（SRD）
収録物名	Journal of reproduction and development
巻(号)	68(5)
ページ	330-334
抄録	Conventional culture systems for bovine embryos are unable to support sustained embryonic development until the developmentally mature blastocyst stage. Although we have previously developed an on-gel culture system that enables bovine blastocysts to complete cell segregation events at day (D) 10 following in vitro culture, the development of D10 blastocysts to term has yet to be achieved. In this study, we attained full-term development of D10 mature blastocysts produced using an on-gel culture system. Two calves derived from on-gel-cultured embryos were vaginally born, showing normal birth and placental weights and no obvious morphological abnormalities. Moreover, we detected no abnormalities in blood metabolic profile analyses. Our findings indicate that on-gel culturing can be used to facilitate the development of developmentally mature blastocysts to term, and produce healthy viable calves. This culture system could make a valuable contribution to cattle production and would enable a of for bovine-specific pre-implantation development.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0916-8818
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2022-054