Fluoridated Apatite Coating on Human Dentin via Laser-Assisted Pseudo-Biomineralization with the Aid of a Light-Absorbing Molecule

Oyane, Ayako; Sakamaki, Ikuko; Nakamura, Maki; Koga, Kenji; Shitomi, Kanako; Tanaka, Saori; Miyaji, Hirofumi

Fluoridated Apatite Coating on Human Dentin via Laser-Assisted Pseudo-Biomineralization with the Aid of a Light-Absorbing Molecule

Oyane, Ayako; Sakamaki, Ikuko; Nakamura, Maki et al.

International Journal of Molecular Sciences, 2022, 23(24), 15981

Permalink : https://hdl.handle.net/2115/87606

このアイテムのアクセス数:325件（2026-01-25 11:56 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/87606
タイトル	Fluoridated Apatite Coating on Human Dentin via Laser-Assisted Pseudo-Biomineralization with the Aid of a Light-Absorbing Molecule
著者	Oyane, Ayako ; Sakamaki, Ikuko ; Nakamura, Maki ; Koga, Kenji ; Shitomi, Kanako ; Tanaka, Saori ; Miyaji, Hirofumi
e-Rad_Researcher 50356672 著者名 Oyane, Ayako
著者名 Sakamaki, Ikuko
e-Rad_Researcher 00568925 著者名 Nakamura, Maki
e-Rad_Researcher 30356969 著者名 Koga, Kenji
e-Rad_Researcher 70881449 著者名 Shitomi, Kanako
e-Rad_Researcher 90344522 著者名 Tanaka, Saori
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
言語	英語
キーワード	apatite
	fluoride
	laser
	biomineralization
	coating
	dentin
	biomimetic process
発行日	2022-12-15
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	23(24)
ページ	15981
抄録	A simple, area-specific coating technique for fluoridated apatite (FAp) on teeth would be useful in dental applications. Recently, we achieved area-specific FAp coating on a human dentin substrate within 30 min by a laser-assisted biomimetic (LAB) process; pulsed Nd:YAG laser irradiation in a fluoride-containing supersaturated calcium phosphate solution (FCP solution). The LAB-processed, FAp-coated dentin substrate exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. In the present study, we refined the LAB process with a combination of a dental diode laser and a clinically approved light-absorbing molecule, indocyanine green (ICG). A micron-thick FAp layer was successfully formed on the dentin surface within only 3 min by the refined LAB process, i.e., dental diode laser irradiation in the FCP solution following ICG treatment. The ICG layer precoated on the dentin substrate played a crucial role in inducing rapid pseudo-biomineralization (FAp layer formation) on the dentin surface by absorbing laser light at the solid-liquid interface. In the refined LAB process, the precoated ICG layer was eliminated and replaced with the newly formed FAp layer composed of vertically oriented pillar-like nanocrystals. Cross-sectional ultrastructural analysis revealed a smooth interface between the FAp layer and the dentin substrate. The refined LAB process has potential as a tool for the tooth surface functionalization and hence, is worth further process refinement and in vitro and in vivo studies.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1422-0067
NCID	AA12038549
関連情報	PMID 36555621
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms232415981