Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae

Kataba, Andrew; Botha, Tarryn L.; Nakayama, Shouta M. M.; Yohannes, Yared B.; Ikenaka, Yoshinori; Wepener, Victor; Ishizuka, Mayumi

Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae

Kataba, Andrew; Botha, Tarryn L.; Nakayama, Shouta M. M. et al.

Comparative biochemistry and physiology Part C: Toxicology & pharmacology, 2022, 252, 109215

Permalink : https://hdl.handle.net/2115/87844

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アクセス権	open access
URI	https://hdl.handle.net/2115/87844
タイトル	Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae
著者	Kataba, Andrew ; Botha, Tarryn L. ; Nakayama, Shouta M. M. ; Yohannes, Yared B. ; Ikenaka, Yoshinori ; Wepener, Victor ; Ishizuka, Mayumi
著者名 Kataba, Andrew
著者名 Botha, Tarryn L.
e-Rad_Researcher 90647629 著者名 Nakayama, Shouta M. M.
著者名 Yohannes, Yared B.
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Wepener, Victor
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	Zebrafish
	Lead
	Cardiovascular
	Apoptosis
	Twitching
	Fish Embryo Test (FET)
発行日	2022-02-01
出版者	Elsevier
収録物名	Comparative biochemistry and physiology Part C: Toxicology & pharmacology
巻(号)	252
ページ	109215
抄録	Early developmental stages of aquatic organisms including fish are inherently vulnerable to lead (Pb) and other water metal contaminants. However, reports on the deleterious effects of environmentally relevant Pb levels are limited. To this end, we exposed 2.5 h post fertilization (hpf) old zebrafish (Danio rerio) embryos to a range of Pb concentrations encompassing environmentally relevant levels (1, 10, 25, 50 and 100 mu g/L Pb) until 96 hpf. Exposure negatively impacted the development and survival of zebrafish embryos by inducing embryo coagulation related mortalities in a concentration-dependent manner. At 24 hpf, the highest level of exposure (100 mu g/ L Pb) had impaired embryo activity characterized by reduced burst activity and the number of movements per minute made by embryos. At 72 hpf, newly hatched larvae exhibited adverse cardiovascular effects (100 mu g/L Pb group) and neuromuscular effects (50 and 100 mu g/L Pb groups). The antioxidant system dysregulation evidenced by downregulation of catalase, and upregulation of mRNA expression of glutathione S-transferase and cytochrome oxidase subunit I were observed. The pro-apoptotic tumour protein P53 (TP53) and the anti-apoptotic B cell lymphoma-2 (Bcl-2) mRNA expression levels were also affected. The former was downregulated across exposed groups and the latter was upregulated and downregulated in the groups with Pb concentrations less than 50 mu g/L Pb and downregulated in 50 mu g/L Pb, respectively. These findings suggest that Pb within environmentally relevant levels may be deleterious to developing zebrafish.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1532-0456
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.cbpc.2021.109215