Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria

Oshiki, Mamoru; Fukushima, Toshikazu; Kawano, Shuichi et al.

Microbes and environments, 2022, 37(1), ME21073

Permalink : https://hdl.handle.net/2115/87919

このアイテムのアクセス数:202件（2026-08-13 06:49 集計）

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1264/jsme2.ME21073			15

アクセス権	open access
URI	https://hdl.handle.net/2115/87919
タイトル	Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria
著者	Oshiki, Mamoru ; Fukushima, Toshikazu ; Kawano, Shuichi ; Nakagawa, Junichi
e-Rad_Researcher 90540865 著者名 Oshiki, Mamoru
著者名 Fukushima, Toshikazu
著者名 Kawano, Shuichi
著者名 Nakagawa, Junichi
言語	英語
キーワード	recombination amplification reaction (RPA)
	endpoint assay
	gene quantification
	thiocyanate dehydrogenase
発行日	2022
出版者	Japanese Society of Microbial Ecology, Department of Bioresource Science
収録物名	Microbes and environments
巻(号)	37(1)
ページ	ME21073
抄録	An endpoint recombination amplification reaction (RPA) assay for assessing the abundance of the gene encoding thiocyanate dehydrogenase (TcDH) in Thiohalobacter has been developed. The RPA reaction was performed at 37 degrees C for 30 min, terminated by the addition of sodium dodecyl sulfate (SDS) solution, and the DNA concentration of the RPA product was fluorometrically measured. The abundance of TcDH in 22 activated sludge samples and 7 thiocyanate-degrading enrichment cultures ranged between 2.5x10(3) and 1.5x10(6) copies mu L-1, showing a linear relationship (R-2=0.83) with those measured using a conventional quantitative PCR assay.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1342-6311
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1264/jsme2.ME21073