Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications

Godigamuwa, Kasun; Nakashima, Kazunori; Tsujitani, Sota et al.

Journal of materials chemistry B, 2023, 11(8), 1654-1658

Permalink : https://hdl.handle.net/2115/88579

このアイテムのアクセス数:252件（2026-08-25 06:18 集計）

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1039/d2tb02581g			34

アクセス権	open access
URI	https://hdl.handle.net/2115/88579
タイトル	Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications
著者	Godigamuwa, Kasun ; Nakashima, Kazunori ; Tsujitani, Sota ; Naota, Ryo ; Maulidin, Ilham ; Kawasaki, Satoru
著者名 Godigamuwa, Kasun
著者名 Nakashima, Kazunori
著者名 Tsujitani, Sota
著者名 Naota, Ryo
著者名 Maulidin, Ilham
e-Rad_Researcher 00304022 著者名 Kawasaki, Satoru
言語	英語
発行日	2023-02-28
出版者	Royal Society of Chemistry
収録物名	Journal of materials chemistry B
巻(号)	11(8)
ページ	1654-1658
抄録	An enzyme-encapsulated silica-based hybrid material was developed using a chitosan gel. Fusion silicatein (InaKC-ChBD-Sil), silicatein fused with a soluble tag and chitin-binding domain, was employed as an interfacial catalyst to form silica on a chitosan gel matrix under physiological conditions, and horseradish peroxidase was immobilised on the hybrid material. Silica formation on the gel was verified via fluorescence microscopy using a designed fusion protein called TBP-mCherry, a fluorescent protein fused with a silica-binding peptide. We report a chitosan gel-silica hybrid material capable of encapsulating enzymes for biomedical and environmental applications.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2050-750X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1039/d2tb02581g