Molecular Characterization of Mutations in Isoniazid- and Rifampicin-Resistant Mycobacterium tuberculosis Isolated in Thailand

Rudeeaneksin, Janisara; Phetsuksiri, Benjawan; Nakajima, Chie; Fukushima, Yukari; Suthachai, Worasak; Tipkrua, Nattakan; Suthum, Krairerk; Jekloh, Nasron; Bunchoo, Supranee; Srisungngam, Sopa; Klayut, Wiphat; Hamada, Shigeyuki; Suzuki, Yasuhiko

Molecular Characterization of Mutations in Isoniazid- and Rifampicin-Resistant Mycobacterium tuberculosis Isolated in Thailand

Rudeeaneksin, Janisara; Phetsuksiri, Benjawan; Nakajima, Chie et al.

Japanese journal of infectious diseases, 2023, 76(1), 39-45

Permalink : https://hdl.handle.net/2115/88640

このアイテムのアクセス数:371件（2026-08-13 07:18 集計）

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https://doi.org/10.7883/yoken.JJID.2022.055			21

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アクセス権	open access
URI	https://hdl.handle.net/2115/88640
タイトル	Molecular Characterization of Mutations in Isoniazid- and Rifampicin-Resistant Mycobacterium tuberculosis Isolated in Thailand
著者	Rudeeaneksin, Janisara ; Phetsuksiri, Benjawan ; Nakajima, Chie ; Fukushima, Yukari ; Suthachai, Worasak ; Tipkrua, Nattakan ; Suthum, Krairerk ; Jekloh, Nasron ; Bunchoo, Supranee ; Srisungngam, Sopa ; Klayut, Wiphat ; Hamada, Shigeyuki ; Suzuki, Yasuhiko
著者名 Rudeeaneksin, Janisara
著者名 Phetsuksiri, Benjawan
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
著者名 Fukushima, Yukari
著者名 Suthachai, Worasak
著者名 Tipkrua, Nattakan
著者名 Suthum, Krairerk
著者名 Jekloh, Nasron
著者名 Bunchoo, Supranee
著者名 Srisungngam, Sopa
著者名 Klayut, Wiphat
著者名 Hamada, Shigeyuki
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Tuberculosis
	rifampicin-resistance
	isoniazid-resistance
	Thailand
発行日	2023-01-24
出版者	国立感染症研究所（National Institute of Infectious Diseases, Japan）
収録物名	Japanese journal of infectious diseases
巻(号)	76(1)
ページ	39-45
抄録	The control of drug-resistant tuberculosis (TB) is a major challenge. The frequency and mutation characteristics indicate the efficiency of molecular tests for the rapid detection of TB drug resistance. This study examined the existence of katG and inhA mutations for isoniazid (INH) resistance and rpoB mutations for rifampicin (RFP) resistance. In total, 178 drug-resistant Mycobacterium tuberculosis (MTB) isolates were analyzed. Mutations in katG encoding and inhA regulatory regions were detected in 136/168 (81.0%) and 29/168 (17.3%), respectively, with the most prominent mutation of Ser315Thr substitution in katG in 126/168 (75.0%), and-15 C to T substitution in the regulatory region of the inhA (26/168; 15.5%). Two distinct katG mutations (Tyr337Cys, 1003InsG) were identified. Of 125 RFP-resistant isolates, 118 (94.4%) carried mutations affecting the 81-bp RFP resistance-determining region, with the most commonly affected codons 450, 445, and 435 identified in 74 (59.2%), 26 (20.8%), and 12 (9.6%) isolates, respectively. Genetic mutations were highly associated with phenotypic INH and RFP resistance, and the majority shared similarities with those reported in previous studies in Thailand and other Asian countries. These data are useful for guiding the use and improvement of molecular tests for TB drug resistance.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1344-6304
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.7883/yoken.JJID.2022.055