Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia

Bwalya, Precious; Solo, Eddie S.; Chizimu, Joseph Y.; Shrestha, Dipti; Mbulo, Grace; Thapa, Jeewan; Nakajima, Chie; Suzuki, Yasuhiko

Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia

Bwalya, Precious; Solo, Eddie S.; Chizimu, Joseph Y. et al.

Tuberculosis, 2022, 133, 102184

Permalink : https://hdl.handle.net/2115/88701

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/88701
タイトル	Characterization of embB mutations involved in ethambutol resistance in multi-drug resistant Mycobacterium tuberculosis isolates in Zambia
著者	Bwalya, Precious ; Solo, Eddie S. ; Chizimu, Joseph Y. ; Shrestha, Dipti ; Mbulo, Grace ; Thapa, Jeewan ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Bwalya, Precious
著者名 Solo, Eddie S.
著者名 Chizimu, Joseph Y.
著者名 Shrestha, Dipti
著者名 Mbulo, Grace
著者名 Thapa, Jeewan
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Mycobacterium tuberculosis
	Ethambutol
	Multi-drug resistance
	embB mutations
	Zambia
発行日	2022-03
出版者	Elsevier
収録物名	Tuberculosis
巻(号)	133
ページ	102184
抄録	Background: Ethambutol (EMB) is an important anti-tuberculosis drug used in the management of multi-drug resistant tuberculosis (MDR-TB). Mutations in embB are the major mechanism of resistance. This study investigated embB mutations among MDR-TB isolates and analyzed their correlations with phenotypic drug susceptibility testing (DST) in Zambia. Method: A total of 132 MDR-TB isolates were collected from January 2014 to April 2017 and characterized using MGIT 960 systems, embB sequencing, and spoligotyping. Results: Out of 61 phenotypically EMB resistant isolates, 53 had mutations in embB. Among the 71 EMB susceptible isolates, 47 had embB mutations. Sensitivity of embB mutations was 86.9% while specificity was 33.8%. CAS1_Kili (SIT21) had high odds of having embB mutations, particularly, G918A (Met306eIl) (Odds ratio 16.7, p < 0.0001). Conclusion: Molecular EMB resistance testing by DNA sequencing can improve detection of EMB resistance among MDR-TB patients in Zambia. Additionally, CAS1_Kili was associated with embB amino acid substitution Met306Ile suggesting transmission. A detailed investigation to track and determine transmission hotspot area for MDR-TB could help optimize control strategies.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1472-9792
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.tube.2022.102184