Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG) n telomeric probe in some species of Lepidoptera

Yoshido, Atsuo; Marec, František; Sahara, Ken

Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG) n telomeric probe in some species of Lepidoptera

Yoshido, Atsuo; Marec, František; Sahara, Ken

Chromosoma, 2005, 114(3), 193-202

Permalink : https://hdl.handle.net/2115/888

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アクセス権	open access
URI	https://hdl.handle.net/2115/888
タイトル	Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG) n telomeric probe in some species of Lepidoptera
著者	Yoshido, Atsuo ; Marec, František ; Sahara, Ken
著者名 Yoshido, Atsuo
著者名 Marec, František
e-Rad_Researcher 30241368 著者名 Sahara, Ken 佐原, 健
言語	英語
キーワード	ｙ-chromosomes
	bombyx-mori
	evolution
	insects
	differentiation
	chromatin
	karyotype
	sequence
	females
	system
発行日	2005-07-14
収録物名	Chromosoma
巻(号)	114(3)
ページ	193-202
抄録	Abstract We have developed a simple method to resolve the sex chromosome constitution in females of Lepidoptera by using a combination of genomic in situ hybridization (GISH) and fluorescence in situ hybridization with (TTAGG)n telomeric probe (telomere-FISH). In pachytene configurations of sex chromosomes, GISH differentiated W heterochromatin and telomere-FISH detected the chromosome ends.With this method we showed that Antheraea yamamai has a standard system with a fully differentiated W–Z sex chromosome pair. In Orgyia antiqua, we confirmed the presence of neo-W and neo-Z chromosomes, which most probably originated by fusion of the ancestral W and Z with an autosome pair. In contrast to earlier data, Orgyia thyellina females displayed a neo-ZW1W2 sex chromosome constitution. A neo-WZ1Z2 trivalent was found in females of Samia cynthia subsp. indet., originating from a population in Nagano, Japan. Whereas another subspecies collected in Sapporo, Japan, and determined as S. cynthia walkeri, showed a neo-W/neo-Z bivalent similar to O. antiqua, and the subspecies S. cynthia ricini showed a Z univalent (a Z/ZZ system). The combination of GISH and telomere-FISH enabled us to acquire not only reliable information about sex chromosome constitution but also an insight into sex chromosome evolution in Lepidoptera.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0009-5915
関連情報	PMID 16021495
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00412-005-0013-9