Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model

Suzuki, Hisataka; Ura, Katsuro; Ukeba, Daisuke et al.

Cells, 2023, 12(3), 505

Permalink : https://hdl.handle.net/2115/88934

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アクセス権
metadata only access
URI
https://hdl.handle.net/2115/88934
タイトル
Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model
著者
Suzuki, Hisataka ; Ura, Katsuro ; Ukeba, Daisuke ; Suyama, Takashi ; Iwasaki, Norimasa ; Watanabe, Masatoki ; Matsuzaki, Yumi ; Yamada, Katsuhisa ; Sudo, Hideki
著者名
Suzuki, Hisataka
著者名
Ura, Katsuro
著者名
Ukeba, Daisuke
著者名
Suyama, Takashi
著者名
Iwasaki, Norimasa
著者名
Watanabe, Masatoki
著者名
Matsuzaki, Yumi
著者名
Yamada, Katsuhisa
e-Rad_Researcher
30374367
著者名
Sudo, Hideki
言語
英語
キーワード
low back pain
intervertebral disc regeneration
ultra-purified clonogenic bone marrow-derived mesenchymal stem cell
ultra-purified alginate
発行日
2023-02
出版者
MDPI
収録物名
Cells
巻(号)
12(3)
ページ
505
抄録
Intervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate (UPAL) gel promotes tissue repair and reduces discogenic pain, and a combination of ultra-purified, Good Manufacturing Practice (GMP)-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs), and the UPAL gel increasingly enhanced IVD regeneration in animal models. This study investigated the therapeutic efficacy of injecting a mixture of REC and UPAL non-gelling solution for discogenic pain and IVD regeneration in a rat caudal nucleus pulposus punch model. REC and UPAL mixture and UPAL alone suppressed not only the expression of TNF-alpha, IL-6, and TrkA (p < 0.01, respectively), but also IVD degeneration and nociceptive behavior compared to punching alone (p < 0.01, respectively). Furthermore, REC and UPAL mixture suppressed these expression levels and nociceptive behavior compared to UPAL alone (p < 0.01, respectively). These results suggest that this minimally invasive treatment strategy with a single injection may be applied to treat discogenic pain and as a regenerative therapy.
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学術雑誌論文
出版タイプ
Version of Record
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出版社版DOI
https://doi.org/10.3390/cells12030505