Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices

Maeki, Masatoshi; Okada, Yuto; Uno, Shuya; Sugiura, Kaisei; Suzuki, Yuichi; Okuda, Kento; Sato, Yusuke; Ando, Masao; Yamazaki, Hiroyuki; Takeuchi, Masaki; Ishida, Akihiko; Tani, Hirofumi; Harashima, Hideyoshi; Tokeshi, Manabu

Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices

Maeki, Masatoshi; Okada, Yuto; Uno, Shuya et al.

Applied materials today, 2023, 31, 101754

Permalink : https://hdl.handle.net/2115/89053

このアイテムのアクセス数:527件（2026-01-17 19:29 集計）

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/89053
タイトル	Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices
著者	Maeki, Masatoshi ; Okada, Yuto ; Uno, Shuya ; Sugiura, Kaisei ; Suzuki, Yuichi ; Okuda, Kento ; Sato, Yusuke ; Ando, Masao ; Yamazaki, Hiroyuki ; Takeuchi, Masaki ; Ishida, Akihiko ; Tani, Hirofumi ; Harashima, Hideyoshi ; Tokeshi, Manabu
著者名 Maeki, Masatoshi
著者名 Okada, Yuto
著者名 Uno, Shuya
著者名 Sugiura, Kaisei
著者名 Suzuki, Yuichi
著者名 Okuda, Kento
e-Rad_Researcher 10735624 著者名 Sato, Yusuke
著者名 Ando, Masao
著者名 Yamazaki, Hiroyuki
著者名 Takeuchi, Masaki
e-Rad_Researcher 20312382 著者名 Ishida, Akihiko
e-Rad_Researcher 10271644 著者名 Tani, Hirofumi
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
e-Rad_Researcher 60311437 著者名 Tokeshi, Manabu
言語	英語
キーワード	Lipid nanoparticles
	mRNA vaccines
	Microfluidic device
	Microfabrication
	Mass production
発行日	2023-04
出版者	Elsevier
収録物名	Applied materials today
巻(号)	31
ページ	101754
抄録	Microfluidic devices are widely used in lipid nanoparticle (LNP)-based vaccines and nanomedicine research. These devices should be stiff enough to withstand the high flow rate for the mass production of LNPs, and malleable enough to use when fabricating complicated microchannel or micromixer structures, such as stag-gering herringbone micromixers. Due to the limitations of the available fabrication methods, optimal micro -fluidic devices have not yet been developed. In this study, we report the development of a glass-based microfluidic device based on the invasive Lipid Nanoparticle Production (iLiNP) device (R) reported previously. The LNP size controllability of glass-based iLiNP device was similar to that of the poly(dimethylsiloxane) (PDMS)-based iLiNP device, and the glass-iLiNP device was used for mRNA-loaded LNP production with ionizable lipids used for COVID-19 mRNA vaccines. We also demonstrate a piling-and numbering-up strategy based on glass-iLiNP device. The iLiNP unit composed of five-layered microchannels was fabricated by piling-up each glass-iLiNP device followed by parallelization (numbering-up) for the mass production of LNPs. This iLiNP system can produce LNPs with sizes ranging between 20 and 60 nm at a flow rate of 20-50 mL/min, and its performance is comparable to that of the commercially available microfluidic system like NanoAssemblr (R).
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2352-9407
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.apmt.2023.101754