The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo

Lau, Alice C. C.; Mohamed, Wessam Mohamed Ahmed; Nakao, Ryo; Onuma, Manabu; Qiu, Yongjin; Nakajima, Nobuyoshi; Shimozuru, Michito; Mohd-Azlan, Jayasilan; Moustafa, Mohamed Abdallah Mohamed; Tsubota, Toshio

The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo

Lau, Alice C. C.; Mohamed, Wessam Mohamed Ahmed; Nakao, Ryo et al.

Microbial Genomics, 2023, 9(2), 954

Permalink : https://hdl.handle.net/2115/89159

このアイテムのアクセス数:642件（2026-01-16 16:16 集計）

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https://doi.org/10.1099/mgen.0.000954

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/89159
タイトル	The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo
著者	Lau, Alice C. C. ; Mohamed, Wessam Mohamed Ahmed ; Nakao, Ryo ; Onuma, Manabu ; Qiu, Yongjin ; Nakajima, Nobuyoshi ; Shimozuru, Michito ; Mohd-Azlan, Jayasilan ; Moustafa, Mohamed Abdallah Mohamed ; Tsubota, Toshio
著者名 Lau, Alice C. C.
著者名 Mohamed, Wessam Mohamed Ahmed
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
著者名 Onuma, Manabu
著者名 Qiu, Yongjin
著者名 Nakajima, Nobuyoshi
e-Rad_Researcher 50507168 著者名 Shimozuru, Michito
著者名 Mohd-Azlan, Jayasilan
著者名 Moustafa, Mohamed Abdallah Mohamed
e-Rad_Researcher 10207441 著者名 Tsubota, Toshio
言語	英語
キーワード	microbiome
	next-generation sequencing
	pathogens
	Sarawak
	ticks
発行日	2023-02-09
出版者	Microbiology Society
収録物名	Microbial Genomics
巻(号)	9(2)
ページ	954
抄録	Tick-borne diseases have recently been considered a potential emerging public health threat in Malaysia; however, fundamental studies into tick-borne pathogens and microbiome appear limited. In this study, six tick species (Ixodes granulatus, Haemaphysa-lis hystricis, Haemaphysalis shimoga, Dermacentor compactus, Dermacentor steini and Dermacentor atrosignatus) collected from two primary forests and an oil palm plantation in Sarawak, Malaysian Borneo, were used for microbiome analysis targeting bacterial 16S rDNA using next-generation sequencing (NGS). In addition, bacterial species were further characterized in con-ventional PCRs to identify potential pathogens. Sequences generated from NGS were first filtered with the Decontam package in R before subsequent microbial diversity analyses. Alpha and beta analyses revealed that the genus Dermacentor had the highest microbial diversity, and H. shimoga significantly differed in microbial composition from other tick species. Alpha and beta diversities were also significantly different between developmental stages of H. shimoga. Furthermore, we observed that some bacterial groups were significantly more abundant in certain tick species and developmental stages of H. shimoga. We tested the relative abundances using pairwise linear discriminant analysis effect size (LEfSe), which also revealed significant microbial composition differences between Borrelia-positive and Borrelia-negative I. granulatus ticks. Finally, pathogenic and potentially pathogenic bacteria circulating in different tick species, such as Rickettsia heilongjiangensis, Ehrlichia sp., Anaplasma sp. and Bartonella spp. were characterized by PCR and sequencing. Moreover, Coxiella and Francisella- like potential symbionts were identified from H. shimoga and D. steini, respectively. More studies are required to unravel the factors associated with the variations observed in this study.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2057-5858
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1099/mgen.0.000954