open access

https://doi.org/10.14943/doctoral.k13043

https://hdl.handle.net/2115/89191

新規抗菌材CPC-Montmorillonite含有粘膜調整材の抗菌性及び生体適合性

英語

biocompatibility

2018-03-22

Hokkaido University

Purpose: The aim of this study was to evaluate the mechanical properties, antimicrobial activity and biocompatibility of a novel antimicrobial tissue conditioner containing cetylpyridinium chloride. Materials and Methods: To examine the mechanical properties of the novel developed material, measurements according to JIS-T6519 and hardness test were carried out. Antimicrobial activity against C. albicans and S. aureus was evaluated. The cell viabilities of fibroblasts and epithelial cells using eluates from materials were measured for cytotoxicity. Also, to assess tissue response, animal experiments were carried out. As control, tissue conditioners which are available on the market were used. Results: On penetration test of JIS-T6519, results of the novel material failed to satisfy the standard. For hardness test, the results were kept on border of other manufactures. In antimicrobial test, the novel tissue conditioner showed antimicrobial activity against C. albicans and S. aureus compared to control materials. This effect was sustained for a week at C. albicans. In the case of S. aureus, the growth of the microbe was suppressed up to 3 weeks. Cell viability of the novel material for the eluate at 1 day was significantly lower than those of control materials for both cells. But the cell viability at 7 days showed no significantly difference. Animal experiments showed that inflammatory responses around materials were not observed on the oral mucosa as control material. Conclusion: Within the limitation of this in vitro and in vivo study, the results suggest that the new developed tissue conditioner containing cetylpyridinium chloride has not only more excellent antimicrobial property, but also same mechanical property and biocompatibility as tissue conditioners on the market.

2018-03-22

甲第13043号

博士(歯学)

(主査) 教授 横山 敦郎, 助教 長谷部 晃, 教授 吉田 靖弘

歯学研究科（口腔医学専攻）

博士論文

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