Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages

Ono, Masataka; Watari, Satomi; Nishizawa-Higashi, Mizuho; Konishi, Tatsuya; Takahashi, Yoshinori; Saeki, Hiroki; Joe, Ga-Hyun

Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages

Ono, Masataka; Watari, Satomi; Nishizawa-Higashi, Mizuho et al.

Food Chemistry: Molecular Sciences, 2023, 6, 100165

Permalink : https://hdl.handle.net/2115/89254

このアイテムのアクセス数:246件（2026-01-24 02:42 集計）

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https://doi.org/10.1016/j.fochms.2023.100165

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/89254
タイトル	Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages
著者	Ono, Masataka ; Watari, Satomi ; Nishizawa-Higashi, Mizuho ; Konishi, Tatsuya ; Takahashi, Yoshinori ; Saeki, Hiroki ; Joe, Ga-Hyun
著者名 Ono, Masataka
著者名 Watari, Satomi
著者名 Nishizawa-Higashi, Mizuho
著者名 Konishi, Tatsuya
著者名 Takahashi, Yoshinori
e-Rad_Researcher 90250505 著者名 Saeki, Hiroki
e-Rad_Researcher 80829052 著者名 Joe, Ga-Hyun
言語	英語
キーワード	Fish water-soluble protein
	Macrophage
	Anti-inflammation
	LPS
	TLR4
	MyD88
発行日	2023
出版者	Elsevier
収録物名	Food Chemistry: Molecular Sciences
巻(号)	6
ページ	100165
抄録	Water-soluble protein (WSP) from fish meat is abundant in the waste effluent generated via the surimi manufacturing process. This study investigated the anti-inflammatory effects and mechanisms of fish WSP using primary macrophages (MO) and animal ingestion. MO were treated with digested-WSP (d-WSP, 500 mu g/mL) with or without lipopolysaccharide (LPS) stimulation. For the ingestion study, male ICR mice (5 weeks old) were fed 4% WSP for 14 days following LPS administration (4 mg/kg body weight). d-WSP decreased the expression of Tlr4, an LPS receptor. Additionally, d-WSP significantly suppressed the secretion of inflammatory cytokines, phagocytic ability, and Myd88 and Il1b expressions of LPS-stimulated macrophages. Furthermore, the ingestion of 4% WSP attenuated not only LPS-induced IL-10 secretion in the blood but also Myd88 and Il1b expressions in the liver. Thus, fish WSP decreases the expressions of the genes involved in the TLR4-MyD88 pathway in MO and the liver, thereby suppressing inflammation.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2666-5662
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.fochms.2023.100165