W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori

Sahara, Ken; Yoshido, Atsuo; Kawamura, Naoko et al.

Chromosoma, 2003, 112(1), 48-55

Permalink : https://hdl.handle.net/2115/893

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アクセス権
open access
URI
https://hdl.handle.net/2115/893
タイトル
W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori
著者
Sahara, Ken ; Yoshido, Atsuo ; Kawamura, Naoko ; Ohnuma, Akio ; Abe, Hiroaki ; Mita, Kazuei ; Oshiki, Toshikazu ; Shimada, Toru ; Asano, Shin-ichiro ; Bando, Hisanori ; Yasukochi, Yuji
e-Rad_Researcher
30241368
著者名
Sahara, Ken
佐原, 健
著者名
Yoshido, Atsuo
著者名
Kawamura, Naoko
著者名
Ohnuma, Akio
著者名
Abe, Hiroaki
著者名
Mita, Kazuei
著者名
Oshiki, Toshikazu
著者名
Shimada, Toru
著者名
Asano, Shin-ichiro
e-Rad_Researcher
20189731
著者名
Bando, Hisanori
著者名
Yasukochi, Yuji
言語
英語
キーワード
comparative genomic hybridization
ephestia-kuehniella lapidoptera
sex-chromosomes
domesticated silkworm
translocation strains
linkage map
dna
chromatin
insects
moth
発行日
2003-06-24
出版者
Springer-Verlag GmbH
収録物名
Chromosoma
巻(号)
112(1)
ページ
48-55
抄録
We isolated four W chromosome-derived bacterial artificial chromosome (W-BAC) clones from Bombyx mori BAC libraries by the polymerase chain reaction and used them as probes for fluorescence in situ hybridization (FISH) on chromosome preparations from B. mori females. All four W-BAC probes surprisingly highlighted the whole wild-type W sex chromosome and also identified the entire original W-chromosomal region in W chromosome-autosome translocation mutants. This is the first successful identification of a single chromosome by means of BAC-FISH in species with holokinetic chromosomes. Genomic in situ hybridization (GISH) by using female-derived genomic probes highlighted the W chromosome in a similar chromosome-painting manner. Besides the W, hybridization signals of W-BAC probes also occurred in telomeric and/or subtelomeric regions of the autosomes. These signals coincided well with those of female genomic probes except one additional GISH signal that was observed in a large heterochromatin block of one autosome pair. Our results support the opinion that the B. mori W chromosome accumulated transposable elements and other repetitive sequences that also occur, but scattered, elsewhere in the respective genome. Edited by: E.R. Schmidt
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The original publication is available at www.springerlink.com
PISSN
0009-5915
EISSN
1432-0886
関連情報
PMID
12827381
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s00412-003-0245-5