Regulation of programmed death ligand 1 expression by interferon-gamma and tumour necrosis factor-alpha in canine tumour cell lines

Owaki, Ryo; Deguchi, Tatsuya; Konnai, Satoru; Maekawa, Naoya; Okagawa, Tomohiro; Hosoya, Kenji; Kim, Sangho; Sunaga, Takafumi; Okumura, Masahiro

Regulation of programmed death ligand 1 expression by interferon-gamma and tumour necrosis factor-alpha in canine tumour cell lines

Owaki, Ryo; Deguchi, Tatsuya; Konnai, Satoru et al.

Veterinary and comparative oncology, 2023

Permalink : https://hdl.handle.net/2115/89335

このアイテムのアクセス数:329件（2026-01-24 01:52 集計）

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https://doi.org/10.1111/vco.12886			2

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/89335
タイトル	Regulation of programmed death ligand 1 expression by interferon-gamma and tumour necrosis factor-alpha in canine tumour cell lines
著者	Owaki, Ryo ; Deguchi, Tatsuya ; Konnai, Satoru ; Maekawa, Naoya ; Okagawa, Tomohiro ; Hosoya, Kenji ; Kim, Sangho ; Sunaga, Takafumi ; Okumura, Masahiro
著者名 Owaki, Ryo
e-Rad_Researcher 10849962 著者名 Deguchi, Tatsuya
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
e-Rad_Researcher 50566156 著者名 Hosoya, Kenji
著者名 Kim, Sangho
e-Rad_Researcher 90649112 著者名 Sunaga, Takafumi
著者名 Okumura, Masahiro
言語	英語
キーワード	cell signalling
	immunology
	in vitro models
	oncology
	small animal
発行日	2023-05-25
出版者	John Wiley & Sons
収録物名	Veterinary and comparative oncology
抄録	Expression of programmed death ligand 1 (PD-L1) on tumour cells provides an immune evasion mechanism by inducing suppression of cytotoxic T cells. Various regulatory mechanisms of PD-L1 expression have been described in human tumours, however, little is known in canine tumours. To investigate whether inflammatory signalling is involved in PD-L1 regulation in canine tumours, the effects of interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha treatment were examined in canine malignant melanoma cell lines (CMeC and LMeC) and an osteosarcoma cell line (HMPOS). The protein level of PD-L1 expression was upregulated by IFN-gamma and TNF-alpha stimulation. Upon IFN-gamma stimulation, all cell lines showed an increase in expression of PD-L1, signal transducer and activator of transcription (STAT)1, STAT3 and genes regulated by STAT activation. Upregulated expression of these genes was suppressed by the addition of a JAK inhibitor, oclacitinib. Contrastingly, upon TNF-alpha stimulation, all cell lines exhibited higher gene expression of the nuclear factor kappa B (NF-kappa B) gene RELA and genes regulated by NF-kappa B activation, whereas expression of PD-L1 was upregulated in LMeC only. Upregulated expression of these genes was suppressed by the addition of an NF-kappa B inhibitor, BAY 11-7082. The expression level of cell surface PD-L1 induced by IFN-gamma and TNF-alpha treatment was reduced by oclacitinib and BAY 11-7082, respectively, indicating that upregulation of PD-L1 expression by IFN-gamma and TNF-alpha stimulation is regulated via the JAK-STAT and NF-kappa B signalling pathways, respectively. These results provide insights into the role of inflammatory signalling in PD-L1 regulation in canine tumours.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1476-5810
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1111/vco.12886