Cattle production by intracytoplasmic sperm injection into oocytes vitrified after ovum pick-up

Kagawa, Shinjiro; Hiraizumi, Shingo; Bai, Hanako; Takahashi, Masashi; Kawahara, Manabu

Cattle production by intracytoplasmic sperm injection into oocytes vitrified after ovum pick-up

Kagawa, Shinjiro; Hiraizumi, Shingo; Bai, Hanako et al.

Theriogenology, 2022, 185, 121-126

Permalink : https://hdl.handle.net/2115/89350

このアイテムのアクセス数:420件（2026-08-11 15:13 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/89350
タイトル	Cattle production by intracytoplasmic sperm injection into oocytes vitrified after ovum pick-up
著者	Kagawa, Shinjiro ; Hiraizumi, Shingo ; Bai, Hanako ; Takahashi, Masashi ; Kawahara, Manabu
著者名 Kagawa, Shinjiro
著者名 Hiraizumi, Shingo
e-Rad_Researcher 60775443 著者名 Bai, Hanako
e-Rad_Researcher 10343964 著者名 Takahashi, Masashi
e-Rad_Researcher 70468700 著者名 Kawahara, Manabu
言語	英語
キーワード	Bovine
	Full-term development
	ICSI
	Oocyte vitrification
	Ovum pick-up
発行日	2022-06
出版者	Elsevier
収録物名	Theriogenology
巻(号)	185
ページ	121-126
抄録	Intracytoplasmic sperm injection (ICSI), oocyte vitrification after ovum pick-up (OPU), and in vitro maturation are reproductive technologies with incredible potential for efficient cattle production. However, the developmental competence of embryos produced by ICSI using vitrified OPU oocytes remains unknown. Here, we aimed to evaluate the developmental competence of these embryos from the early embryo period to full term. The cleavage rate in the ICSI embryos using vitrified OPU oocytes during in vitro culture was significantly lower than those in control in vitro fertilized (IVF) embryos using fresh OPU oocytes (30.9 +/- 4.5% v.s. 65.9 +/- 7.0%) (P < 0.05), but the proportion of blastocysts to cleaved embryos was significantly higher than those of IVF embryos using vitrified OPU oocytes (55.9 +/- 10.8% v.s. 23.2 +/- 9.3%) (P < 0.05). To further investigate the transcription levels of genes related to cell differentiation in ICSI embryos using vitrified OPU oocytes, the relative abundance of mRNAs (OCT4, NANOG, SOX2, CDX2, GATA3, and IFNT) was analyzed by quantitative reverse-transcription PCR. There were no significant differences in the expression levels between ICSI embryos using vitrified OPU oocytes and control IVF embryos. Finally, developmental competence to term in ICSI embryos using vitrified OPU oocytes was examined by embryo transfer, and two healthy calves were born. These findings confirmed that ICSI and vitrification decrease developmental rates in vitro, but both procedures can lead to full-term development of bovine embryos. These results demonstrate that ICSI embryos using vitrification OPU oocytes are viable for cattle production.(c) 2022 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0093-691X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.theriogenology.2022.03.022