Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi

Kapalamula, Thoko Flav; Thapa, Jeewan; Hayashida, Kyoko; Chizimu, Joseph; Tanomsridachchai, Wimonrat; Nyenje, Mirriam Ethel; Mkakosya, Rajab; Nakajima, Chie; Suzuki, Yasuhiko

Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi

Kapalamula, Thoko Flav; Thapa, Jeewan; Hayashida, Kyoko et al.

Journal of Veterinary Diagnostic Investigation, 2023, 35(3), 307-310

Permalink : https://hdl.handle.net/2115/89476

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/89476
タイトル	Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi
著者	Kapalamula, Thoko Flav ; Thapa, Jeewan ; Hayashida, Kyoko ; Chizimu, Joseph ; Tanomsridachchai, Wimonrat ; Nyenje, Mirriam Ethel ; Mkakosya, Rajab ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Kapalamula, Thoko Flav
著者名 Thapa, Jeewan
著者名 Hayashida, Kyoko
著者名 Chizimu, Joseph
著者名 Tanomsridachchai, Wimonrat
著者名 Nyenje, Mirriam Ethel
著者名 Mkakosya, Rajab
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	bovine tuberculosis
	crude extracted DNA
	dry LAMP assay
	Mycobacterium bovis
発行日	2023-04-08
出版者	SAGE Publications
収録物名	Journal of Veterinary Diagnostic Investigation
巻(号)	35(3)
ページ	307-310
抄録	The lack of quick, accurate, and low-cost detection methods has hindered the active control strategies for bovine tuberculosis (bTB) in resource-limited countries with a high burden of disease. We developed a dry loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Mycobacterium bovis, the principal causative agent of bTB, and evaluated the efficacy of the assay using suspected bTB samples collected during routine meat inspection at major regional abattoirs in Malawi. Template genomic DNA was extracted directly from the granulomatous bTB-like lesion (crude extracted DNA), as well as growth from the incubated mycobacterial growth indicator tubes (MGIT). Field results were visualized by the naked eye within 40 min following a color change of the amplified products. The sensitivity and specificity of the dry LAMP assay while using 152 DNA samples extracted from MGIT with confirmed M. bovis results were 98% and 88%, respectively. When 43 randomly selected crude DNA samples from lesions were used, the sensitivity and specificity of the dry LAMP assay were 100% and 75%, respectively. Our LAMP assay offers the potential to meet the demands for a low-cost and rapid field detection tool for bTB in resource-limited countries in which bTB is endemic.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Kapalamula TF, Thapa J, Hayashida K, et al. Direct detection of Mycobacterium bovis by a dry loop-mediated isothermal amplification assay in cattle samples collected during routine abattoir examination in Malawi. Journal of Veterinary Diagnostic Investigation. 35(3) pp.307-310. Copyright © 2023 doi:10.1177/10406387231164596
PISSN	1040-6387
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1177/10406387231164596