Changes in AMPK alpha and Ubiquitin Ligases in Myocyte Reverse Remodeling after Surgical Ventricular Reconstruction in rats with ischemic cardiomyopathy

Shingu, Yasushige; Hieda, Tetsuya; Sugimoto, Satoshi; Asai, Hidetsugu; Yamakawa, Tomoji; Wakasa, Satoru

Changes in AMPK alpha and Ubiquitin Ligases in Myocyte Reverse Remodeling after Surgical Ventricular Reconstruction in rats with ischemic cardiomyopathy

Shingu, Yasushige; Hieda, Tetsuya; Sugimoto, Satoshi et al.

Molecular Biology Reports, 2022, 49(6), 4885-4892

Permalink : https://hdl.handle.net/2115/89789

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アクセス権	open access
URI	https://hdl.handle.net/2115/89789
タイトル	Changes in AMPK alpha and Ubiquitin Ligases in Myocyte Reverse Remodeling after Surgical Ventricular Reconstruction in rats with ischemic cardiomyopathy
著者	Shingu, Yasushige ; Hieda, Tetsuya ; Sugimoto, Satoshi ; Asai, Hidetsugu ; Yamakawa, Tomoji ; Wakasa, Satoru
e-Rad_Researcher 30617064 著者名 Shingu, Yasushige
著者名 Hieda, Tetsuya
著者名 Sugimoto, Satoshi
著者名 Asai, Hidetsugu
著者名 Yamakawa, Tomoji
e-Rad_Researcher 10374365 著者名 Wakasa, Satoru
言語	英語
キーワード	Atrogin-1
	Murf-1
	AMPK alpha
	Myocyte reverse remodeling
	Surgical ventricular reconstruction
発行日	2022-06-01
出版者	Springer
収録物名	Molecular Biology Reports
巻(号)	49(6)
ページ	4885-4892
抄録	Background The change in myocardial protein degradation systems after ventricular unloading has been unknown. We aimed to evaluate the anti-hypertrophic protein adenosine monophosphate-activated protein kinase (AMPK) and two major protein degradation systems (ubiquitin proteasome system and autophagy) in a model of surgical ventricular reconstruction (SVR) in rats with ischemic cardiomyopathy. Methods and Results Rats were randomized into the following groups: sham/sham (control group), myocardial infarction (MI)/sham (sham group) and MI/SVR (SVR group), with an interval of 4 weeks. Two (early, n = 5 for each) and 28 days (late, n=5 for each) after SVR, ventricular size, and wall stress were assessed. Myocyte area, protein expression of AMPK alpha and autophagy markers, and gene expression of ubiquitin ligases (Atrogin-1 and Murf-1) were evaluated in the late phase. In the early phase, left ventricular dimensions and wall stress were smaller in the SVR group than in the sham group, whereas they were comparable in the late period. Myocyte area in the SVR group was reduced to the value in the control group, while it was larger in the sham group than in the control group. Total-AMPK alpha, p-AMPK alpha, and AMPK alpha phosphorylation rates were higher, and Atrogin-1 and Murf-1 were lower in the SVR group than in the sham group, while the autophagy markers were not different between the groups. p-AMPK alpha had strong negative correlations with myocyte area, Atrogin-1, and Murf-1. Conclusions In myocyte reverse remodeling after SVR, AMPK alpha phosphorylation increased in association with reduced gene expression of ubiquitin ligases.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s11033-022-07347-8
PISSN	0301-4851
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11033-022-07347-8