Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid

Nimako, Collins; Ikenaka, Yoshinori; Akoto, Osei; Fujioka, Kazutoshi; Taira, Kumiko; Arizono, Koji; Kato, Keisuke; Takahashi, Keisuke; Nakayama, Shouta M. M.; Ichise, Takahiro; Ishizuka, Mayumi

Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid

Nimako, Collins; Ikenaka, Yoshinori; Akoto, Osei et al.

Journal of Chromatography A, 2021, 1652, 462350

Permalink : https://hdl.handle.net/2115/90325

このアイテムのアクセス数:348件（2026-03-05 13:39 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
20210603_Tissue distribution Manuscript_col-reviewed_vr4	pdf	1.21 MB	341
Highlights (7)	pdf	102 KB	99	Highlights
Suplimentary Data (2)	pptx	734 KB	66	Suplimentary Data

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/90325
タイトル	Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid
著者	Nimako, Collins ; Ikenaka, Yoshinori ; Akoto, Osei ; Fujioka, Kazutoshi ; Taira, Kumiko ; Arizono, Koji ; Kato, Keisuke ; Takahashi, Keisuke ; Nakayama, Shouta M. M. ; Ichise, Takahiro ; Ishizuka, Mayumi
著者名 Nimako, Collins
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Akoto, Osei
著者名 Fujioka, Kazutoshi
e-Rad_Researcher 10163148 著者名 Taira, Kumiko
e-Rad_Researcher 70128148 著者名 Arizono, Koji
e-Rad_Researcher 80276609 著者名 Kato, Keisuke
著者名 Takahashi, Keisuke
e-Rad_Researcher 90647629 著者名 Nakayama, Shouta M. M.
著者名 Ichise, Takahiro
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	Imidacloprid
	IMI-olefin
	4-Hydroxy-IMI
	N-Desnitro-IMI
	5-Hydroxy-IMI
	N-Desnitro-4,5-dehydro-IMI
発行日	2021-08-30
出版者	Elsevier
収録物名	Journal of Chromatography A
巻(号)	1652
ページ	462350
抄録	This study aimed to (i) develop a sensitive method for simultaneous detection and quantification of imidacloprid (IMI) and seven of its metabolites in tissue specimens, and to (ii) determine the biodistribution of the IMI compounds in tissues of C57BL/6J male mice; after exposure to 0.6 mg/kg bw/day of IMI (10% of no observable adverse effect level of IMI) through a powdered diet for 24 weeks. We successfully developed a method which was accurate (recoveries were >= 70% for most compounds), sensitive (LODs <= 0.47 ng/mL and LOQs <= 1.43 ng/mL were recorded for all detected compounds, R-2 >= 0.99) and precise (RSDs <= 20%) for routine analysis of IMI and seven of its metabolites in blood and various tissue matrices. After bio-distributional analysis, IMI and five of its metabolites were detected in mice. Brain, testis, lung, kidney, inguinal white adipose tissue and gonadal white adipose tissue mainly accumulated IMI, blood and mesenteric white adipose tissue mainly accumulated IMI-olefin; liver mainly accumulated desnitroIMI; pancreas predominately accumulated 4-hydroxy-IMI. The desnitro-dehydro-IMI and the desnitro-IMI metabolites recorded tissue-blood concentration ratios >= 1.0 for testis, brain, lung and kidney. The cumulative levels of the six detected IMI compounds (Sigma 6 IMI compounds) were found in the decreasing order: blood > testis > brain > kidney > lung > iWAT > gWAT > mWAT > liver > pancreas. Altogether, this study provided essential data needed for effective mechanistic elucidation of compound-specific adverse outcomes associated with chronic exposures to IMI in mammalian species. (C) 2021 Elsevier B.V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© <2021>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0021-9673
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.chroma.2021.462350