Histochemical examination of blood vessels in murine femora with intermittent PTH administration

Maruoka, Haruhi; Zhao, Shen; Yoshino, Hirona; Abe, Miki; Yamamoto, Tomomaya; Hongo, Hiromi; Haraguchi-Kitakamae, Mai; Nasoori, Alireza; Ishizu, Hotaka; Nakajima, Yuhi; Omaki, Masayuki; Shimizu, Tomohiro; Iwasaki, Norimasa; de Freitas, Paulo Henrique Luiz; Li, Minqi; Hasegawa, Tomoka

Histochemical examination of blood vessels in murine femora with intermittent PTH administration

Maruoka, Haruhi; Zhao, Shen; Yoshino, Hirona et al.

Journal of oral biosciences, 2022, 64(3), 329-336

Permalink : https://hdl.handle.net/2115/90422

このアイテムのアクセス数:890件（2026-01-17 01:25 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/90422
タイトル	Histochemical examination of blood vessels in murine femora with intermittent PTH administration
著者	Maruoka, Haruhi ; Zhao, Shen ; Yoshino, Hirona ; Abe, Miki ; Yamamoto, Tomomaya ; Hongo, Hiromi ; Haraguchi-Kitakamae, Mai ; Nasoori, Alireza ; Ishizu, Hotaka ; Nakajima, Yuhi ; Omaki, Masayuki ; Shimizu, Tomohiro ; Iwasaki, Norimasa ; de Freitas, Paulo Henrique Luiz ; Li, Minqi ; Hasegawa, Tomoka
e-Rad_Researcher 40962577 著者名 Maruoka, Haruhi
著者名 Zhao, Shen
著者名 Yoshino, Hirona
著者名 Abe, Miki
e-Rad_Researcher 50928299 著者名 Yamamoto, Tomomaya
e-Rad_Researcher 00778970 著者名 Hongo, Hiromi
著者名 Haraguchi-Kitakamae, Mai
e-Rad_Researcher 70910909 著者名 Nasoori, Alireza
著者名 Ishizu, Hotaka
著者名 Nakajima, Yuhi
著者名 Omaki, Masayuki
e-Rad_Researcher 60784246 著者名 Shimizu, Tomohiro
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
著者名 de Freitas, Paulo Henrique Luiz
e-Rad_Researcher 60447612 著者名 Li, Minqi
e-Rad_Researcher 50739349 著者名 Hasegawa, Tomoka
言語	英語
キーワード	Blood vessels
	Perivascular stromal cells
	Parathyroid hormone (PTH)
	Endomucin
	a SMA
発行日	2022-09-19
出版者	Elsevier
収録物名	Journal of oral biosciences
巻(号)	64(3)
ページ	329-336
抄録	Objective: To verify the biological effects of parathyroid hormone (PTH) on the blood vessels in the bone, this study aimed to investigate histological alterations in endomucin-positive blood vessels and perivascular cells in murine femora after intermittent PTH administration. For comparison with blood vessels in the bone, we examined the distribution of endomucin-positive blood vessels and surrounding aSMAimmunoreactive perivascular cells in the liver, kidney, and aorta with or without PTH administration. Methods: Six-week-old male C57BL/6J mice received hPTH [1-34] or vehicle for two weeks. All mice were fixed with a paraformaldehyde solution after euthanasia, and the right femora, kidney, liver, and aorta were extracted for immunohistochemical analysis of endomucin, aSMA, ephrinB2, EphB4, and HIF1a. Light microscopic observations of semi-thin sections and transmission electron microscopic (TEM) observations of ultra-thin sections were performed on the left femora. Results: After intermittent PTH administration, aSMA-reactive/ephrinB2-positive stromal cells appeared around endomucin-positive/EphB4-immunoreactive blood vessels in the bone. In addition, intense immunoreactivities of EphB4 and HIF1a were seen in vascular endothelial cells after the PTH treatment. Several stromal cells surrounding PTH-treated blood vessels exhibited well-developed rough endoplasmic reticulum under TEM observations. In contrast to bone tissues, aSMA-positive stromal cells did not increase around the endomucin-positive blood vessels in the kidney, liver, or aorta, even after PTH administration. Conclusion: These findings show that intermittent PTH administration increases aSMA-reactive/ephrinB2-positive perivascular stromal cells in bone tissue but not in the kidney, liver, or aorta, suggesting that PTH preferentially affects blood vessels in the bone. (c) 2022 Published by Elsevier B.V. on behalf of Japanese Association for Oral Biology.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1349-0079
関連情報	PMID 35584768
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.job.2022.05.003