Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants

Kawakubo, Ayaka; Gallois, Jean-Luc; Nakahara, Kenji S.

Journal of general plant pathology, 2022, 89, 47-52

Permalink : https://hdl.handle.net/2115/90544

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アクセス権
open access
URI
https://hdl.handle.net/2115/90544
タイトル
Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants
著者
Kawakubo, Ayaka ; Gallois, Jean-Luc ; Nakahara, Kenji S.
著者名
Kawakubo, Ayaka
著者名
Gallois, Jean-Luc
e-Rad_Researcher
90315606
著者名
Nakahara, Kenji S.
言語
英語
キーワード
iLOV
Cucumber mosaic virus
In vivo monitoring viral spread
発行日
2022-10-12
出版者
Springer
収録物名
Journal of general plant pathology
巻(号)
89
ページ
47-52
抄録
Tracking virus infection in plants is useful to assess plant susceptibility or resistance. The recombinant cucumber mosaic virus (CMV) expressing a 2b-fused green fluorescent protein (GFP, 25 kDa) gradually loses (within weeks) the GFP gene in plants. Here, we constructed CMV expressing a flavin-based small fluorescent protein (iLOV, 10 kDa). CMV-iLOV still retains iLOV after 28 dpi in upper leaves of inoculated Nicotiana benthamiana and allows long-term monitoring of its distribution in plants. Using CMV-iLOV, we showed that pot1, a recessive allele for resistance to potyviruses, does not confer CMV resistance in tomato.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10327-022-01100-6
PISSN
1345-2630
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s10327-022-01100-6