Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx mori

Kawamura, N.; Sahara, K.; Fugo, H.

Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx mori

Kawamura, N.; Sahara, K.; Fugo, H.

Journal of Insect Physiology, 2003, 49(1), 25-30

Permalink : https://hdl.handle.net/2115/908

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アクセス権	open access
URI	https://hdl.handle.net/2115/908
タイトル	Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx mori
著者	Kawamura, N. ; Sahara, K. ; Fugo, H.
著者名 Kawamura, N.
e-Rad_Researcher 30241368 著者名 Sahara, K. 佐原, 健
著者名 Fugo, H.
言語	英語
キーワード	Silkworm
	In vitro culture
	Eupyrene and apyrene sperm
	20-Hydroxyecdysone
	Glucose
発行日	2003-02-08
出版者	Elsevier Science Ltd.
収録物名	Journal of Insect Physiology
巻(号)	49(1)
ページ	25-30
抄録	Two types of sperm, nucleate eupyrene and anucleate apyrene, occur in the silkworm as in other lepidopteran species. Hormones and other substances have been assumed to play important roles in sperm dimorphism. We established an in vitro cultivation system for silkworm spermatocytes, and found that apyrene sperm are not produced when spermatocytes from larval testes are cultivated, though eupyrene spermatocytes develop normally into mature sperm. Based on the fact that ecdysteroid titers increase rapidly and peak 1 day after spinning, and that the amount of glycogen reaches its peak 1 day before the spinning stage, we studied the effects of adding glucose and/or 20-hydroxyecdysone to the culture medium. The experiments disclosed a significant additive effect of both substances on apyrene sperm production.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0022-1910
関連情報	URI http://www.sciencedirect.com/science/journal/00221910
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/S0022-1910(02)00223-8