Methylprednisolone pulse-enhanced neutrophil extracellular trap formation in mice with imiquimod-induced lupus-like disease, resulting in ischaemia of the femoral head cartilage

Ogawa, Hodaka; Yokota, Shunichi; Hosoi, Yumeka; Shindo, Ayano; Ogawa, Naho; Yamamura, Ryodai; Shimizu, Tomohiro; Nakade, Issei; Arai, Suishin; Taniguchi, Mai; Nishibata, Yuka; Masuda, Sakiko; Nakazawa, Daigo; Tomaru, Utano; Iwasaki, Norimasa; Ishizu, Akihiro

Methylprednisolone pulse-enhanced neutrophil extracellular trap formation in mice with imiquimod-induced lupus-like disease, resulting in ischaemia of the femoral head cartilage

Ogawa, Hodaka; Yokota, Shunichi; Hosoi, Yumeka et al.

Lupus Science & Medicine, 2023, 10(2), e001042

Permalink : https://hdl.handle.net/2115/91026

このアイテムのアクセス数:591件（2026-01-18 16:43 集計）

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https://doi.org/10.1136/lupus-2023-001042

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/91026
タイトル	Methylprednisolone pulse-enhanced neutrophil extracellular trap formation in mice with imiquimod-induced lupus-like disease, resulting in ischaemia of the femoral head cartilage
著者	Ogawa, Hodaka ; Yokota, Shunichi ; Hosoi, Yumeka ; Shindo, Ayano ; Ogawa, Naho ; Yamamura, Ryodai ; Shimizu, Tomohiro ; Nakade, Issei ; Arai, Suishin ; Taniguchi, Mai ; Nishibata, Yuka ; Masuda, Sakiko ; Nakazawa, Daigo ; Tomaru, Utano ; Iwasaki, Norimasa ; Ishizu, Akihiro
著者名 Ogawa, Hodaka
著者名 Yokota, Shunichi
著者名 Hosoi, Yumeka
著者名 Shindo, Ayano
著者名 Ogawa, Naho
e-Rad_Researcher 40880000 著者名 Yamamura, Ryodai
e-Rad_Researcher 60784246 著者名 Shimizu, Tomohiro
著者名 Nakade, Issei
著者名 Arai, Suishin
著者名 Taniguchi, Mai
著者名 Nishibata, Yuka
e-Rad_Researcher 10763617 著者名 Masuda, Sakiko
e-Rad_Researcher 60724135 著者名 Nakazawa, Daigo
e-Rad_Researcher 20360901 著者名 Tomaru, Utano
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
e-Rad_Researcher 60321957 著者名 Ishizu, Akihiro
言語	英語
発行日	2023-12-28
収録物名	Lupus Science & Medicine
巻(号)	10(2)
ページ	e001042
抄録	Objectives: Methylprednisolone (mPSL) pulse therapy is an essential option for patients with active systemic lupus erythematosus, but there is a risk of adverse events related to microcirculation disorders, including idiopathic osteonecrosis of the femoral head (ONFH). Recent studies have revealed that excessive neutrophil extracellular traps (NETs) are involved in microcirculation disorders. This study aimed to demonstrate that mPSL pulse could induce NETs in lupus mice and identify the factors contributing to this induction. Methods: Six mice with imiquimod (IMQ)-induced lupus-like disease and six normal mice were intraperitoneally injected with mPSL on days 39 to 41, and five mice with IMQ-induced lupus-like disease and six normal mice were injected with phosphate-buffered saline. Pathological examinations were conducted to evaluate the ischaemic state of the femoral head and tissue infiltration of NET-forming neutrophils. Proteome analysis was performed to extract plasma proteins specifically elevated in mPSL-administered mice with IMQ-induced lupus-like disease, and their effects on NET formation were assessed in vitro. Results: Mice with IMQ-induced lupus-like disease that received mPSL pulse demonstrated ischaemia of the femoral head cartilage with tissue infiltration of NET-forming neutrophils. Proteome analysis suggested that prenylcysteine oxidase 1 (PCYOX1) played a role in this phenomenon. The reaction of PCYOX1-containing very low-density lipoproteins (VLDL) with its substrate farnesylcysteine (FC) induced NETs in vitro. The combined addition of IMQ and mPSL synergistically enhanced VLDL-plus-FC-induced NET formation. Conclusion: PCYOX1 and related factors are worthy of attention to understand the underlying mechanisms and create novel therapeutic strategies for mPSL-mediated microcirculation disorders, including ONFH.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
EISSN	2053-8790
関連情報	PMID 38154828
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1136/lupus-2023-001042