open access

https://hdl.handle.net/2115/91296

Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis

英語

DAPK3

2023-03-13

John Wiley & Sons

FEBS Letters

597(5)

643-656

Stiffness of the extracellular matrix regulates various biological responses, but the response mechanisms are poorly understood. Here, we found that the nuclear diphosphorylated myosin regulatory light chain (2P-MRLC) is a critical mechanomediator that suppresses apoptosis in response to substrate stiffness. Stiff substrates promoted the nuclear localization of 2P-MRLC. Zipper-interacting protein kinase [ZIPK; also known as death-associated protein kinase 3 (DAPK3)], a kinase for MRLC, was localized in the nucleus in response to stiff substrates and promoted the nuclear localization of 2P-MRLC. Moreover, actin fiber formation induced by substrate stiffness promoted the nuclear localization of 2P-MRLC via ZIPK. 2P-MRLC in response to substrate stiffness suppressed the expression of MAF bZIP transcription factor B (MafB) and repressed apoptosis. These findings reveal a newly identified role of MRLC in mechanotransduction.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Onishi, K., Ishihara, S., Takahashi, M., Sakai, A., Enomoto, A., Suzuki, K. and Haga, H. (2023), Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis. FEBS Lett, 597: 643-656., which has been published in final form at Link to final article using the https://doi.org/10.1002/1873-3468.14592. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

0014-5793