open access

https://doi.org/10.14943/doctoral.k13475

https://hdl.handle.net/2115/91600

マウス歯胚の象牙芽細胞層におけるpodoplanin, CD44およびendomucinの局在

英語

CD44

2019-03-25

Hokkaido University

20p

In this study, we attempted to localize the immunoreactivities of podoplanin and CD44, a counterpart possessing a high affinity to podoplanin, as well as endomucin-immunoreactive blood vessels in the regions of odontoblast layers and the underlying sub-odontoblastic layers in murine tooth germs. Endomucin-reactive small blood vessels were scattered throughout the dental papillae of the tooth germs at postnatal day 1 but came to be localized close to the odontoblast/sub-odontoblastic layers until day 3. After postnatal day 5, small blood vessels were seen forming in odontoblast cell layers, while blood vessels with relatively larger diameters were seen forming in sub-odontoblastic layers. Immunoreactivities of podoplanin and CD44 were not detectable in the cells of dental papillae facing the inner enamel epithelium at postnatal day 1. However, at around postnatal days 3?5, podoplanin was localized in the odontoblast layer but not in the sub-odontoblastic layer, whereas CD44 was observed in the sub-odontoblastic layer but not in the odontoblast layer. The exclusive immunolocalization of podoplanin and CD44 in the odontoblast layers and sub-odontoblastic layers was seen after postnatal day 3 of the tooth germs, which is when the mesenchymal cells of dental papillae have already differentiated into mature odontoblasts at the cusp tip. Taken together, it seems likely that endomucin-reactive small blood vessels extended to the podoplanin-positive odontoblast layers, whereas endomucin-reactive large blood vessels were already present in CD44-immmunopositive sub-odontoblastic layer, indicating the cellular regulation on the vascularization of endomucin-reactive endothelial cells during odontogenesis of the tooth germs.

2019-03-25

甲第13475号

博士(歯学)

(主査) 教授 八若 保孝, 教授 網塚 憲生, 教授 土門 卓文, 助教 長谷川 智香

歯学研究科(口腔医学専攻)

博士論文

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