Rapid and Reliable Steatosis Rat Model Shrsp5-Dmcr for Cold Storage Experiment

Fukai, Moto; Sugimori, Hiroyuki; Sakamoto, Sodai; Shibata, Kengo; Kameda, Hiroyuki; Ishikawa, Takahisa; Kawamura, Norio; Fujiyoshi, Masato; Fujiyoshi, Sunao; Kudo, Kohsuke; Shimamura, Tsuyoshi; Taketomi, Akinobu

Rapid and Reliable Steatosis Rat Model Shrsp5-Dmcr for Cold Storage Experiment

Fukai, Moto; Sugimori, Hiroyuki; Sakamoto, Sodai et al.

Transplantation proceedings, 2023, 55(4), 1032-1035

Permalink : https://hdl.handle.net/2115/92185

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アクセス権	open access
URI	https://hdl.handle.net/2115/92185
タイトル	Rapid and Reliable Steatosis Rat Model Shrsp5-Dmcr for Cold Storage Experiment
著者	Fukai, Moto ; Sugimori, Hiroyuki ; Sakamoto, Sodai ; Shibata, Kengo ; Kameda, Hiroyuki ; Ishikawa, Takahisa ; Kawamura, Norio ; Fujiyoshi, Masato ; Fujiyoshi, Sunao ; Kudo, Kohsuke ; Shimamura, Tsuyoshi ; Taketomi, Akinobu
e-Rad_Researcher 60374344 著者名 Fukai, Moto
著者名 Sugimori, Hiroyuki
著者名 Sakamoto, Sodai
著者名 Shibata, Kengo
著者名 Kameda, Hiroyuki
著者名 Ishikawa, Takahisa
著者名 Kawamura, Norio
著者名 Fujiyoshi, Masato
著者名 Fujiyoshi, Sunao
著者名 Kudo, Kohsuke
著者名 Shimamura, Tsuyoshi
e-Rad_Researcher 70363364 著者名 Taketomi, Akinobu
言語	英語
発行日	2023-05
出版者	Elsevier
収録物名	Transplantation proceedings
巻(号)	55(4)
ページ	1032-1035
抄録	Interventions for liver grafts with moderate macrovesicular steatosis have been important in enlarging donor pools. Here, we tested a high-fat and cholesterol (HFC) diet to create a steatosis model for cold hepatic preservation and reperfusion experiments. The aim of the present study was to assess the steatosis model's reliability and to show the resulting graft's quality for cold preservation and reperfusion experiment. Male SHRSP5-Dmcr rats were raised with an HFC diet for up to 2 weeks. The fat content was evaluated using magnetic resonance imaging (MRI) proton density fat fraction (PDFF). The nonalcoholic fatty liver disease activity score (NAS) was evaluated after excision. Steatosis created by 2 weeks of HFC diet was subjected to 24-hour cold storage in the University of Wisconsin and the original test solution (new sol.). Grafts were applied to isolated perfused rat livers for simulating reperfusion. The NAS were 2.2 (HFC 5 days), 3.3 (HFC 1 week), and 5.0 (HFC 2 weeks). Ballooning and fibrosis were not observed in any group. An MRI-PDFF showed 0.2 (HFC 0 days), 12.0 (HFC 1 week), and 18.9 (HFC 2 weeks). The NAS and MRI-PDFF values correlated. Many indices in the isolated perfused rat liver experiment tended to improve in the new sol. group but were insufficient. Although the new sol. failed to be effective, it acted at multiple sites under difficult conditions. In conclusion, the HFC diet for 2 weeks in SHRSP5-Dmcr rats, together with MRI-PDFF evaluation, is a reliable method for creating simple steatosis and provides good-quality cold preservation and reperfusion experiments.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0041-1345
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.transproceed.2023.02.063