open access

https://hdl.handle.net/2115/92467

Selective Detection of Intracellular Drug Metabolism by Metal-Organic Framework-Coated Plasmonic Nanowire

英語

surface-enhanced Raman scattering

2023-05-21

Wiley-Blackwell

Advanced optical materials

Unveiling intracellular drug metabolism is crucial for improving drug development, which requires real-time detection with molecular selectivity in the intracellular environment. Surface-enhanced Raman scattering (SERS) with metal nanoparticles enables the detection of molecules in living cells, but after entering the cells, most nanoparticles are captured into vesicles, limiting the SERS detection inside these compartments. Moreover, the identification of the target signal in the complex intracellular environment is challenging due to Raman fingerprints from endogenous material interfering with the drug signal. To overcome these issues, here the coating of a silver nanowire with zeolitic imidazolate framework-8 (ZIF-8) as a novel endoscopic probe with molecular selectivity to investigate the location and metabolism in cells of a common anticancer drug, irinotecan, is reported. Irinotecan in cells is metabolized by carboxylesterase to form SN-38, which inhibits topoisomerase I and DNA synthesis. Thanks to the molecular selectivity of ZIF-8, the endoscopic probe selectively adsorbs and detects SERS signal of SN-38 over irinotecan. This selectivity enables monitoring of the conversion of irinotecan into SN-38 and following its intracellular location over time. This work clearly shows the potential of metal-organic framework-coated nanowire endoscopy to specifically track drug molecules and explore their metabolism in cells.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Q. Zhang, T. Murasugi, K. Watanabe, H. Wen, Y. Tian, M. Ricci, S. Rocha, T. Inose, H. Kasai, F. Taemaitree, H. Uji-i, K. Hirai, B. Fortuni, Selective Detection of Intracellular Drug Metabolism by Metal-Organic Framework-Coated Plasmonic Nanowire. Adv. Optical Mater. 2023, 11, 2300856. , which has been published in final form at https://doi.org/10.1002/adom.202300856. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

2195-1071