Monocarboxylate Transporters 1 and 2 Are Responsible for L-Lactate Uptake in Differentiated Human Neuroblastoma SH-SY5Y Cells

Sakuma, Tomoya; Mukai, Yuto; Yamaguchi, Atsushi; Suganuma, Yudai; Okamoto, Keisuke; Furugen, Ayako; Narumi, Katsuya; Ishikawa, Shuhei; Saito, Yoshitaka; Kobayashi, Masaki

Monocarboxylate Transporters 1 and 2 Are Responsible for L-Lactate Uptake in Differentiated Human Neuroblastoma SH-SY5Y Cells

Sakuma, Tomoya; Mukai, Yuto; Yamaguchi, Atsushi et al.

Biological & pharmaceutical bulletin, 2024, 47(4), 764-770

Permalink : https://hdl.handle.net/2115/92588

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アクセス権	open access
URI	https://hdl.handle.net/2115/92588
タイトル	Monocarboxylate Transporters 1 and 2 Are Responsible for L-Lactate Uptake in Differentiated Human Neuroblastoma SH-SY5Y Cells
著者	Sakuma, Tomoya ; Mukai, Yuto ; Yamaguchi, Atsushi ; Suganuma, Yudai ; Okamoto, Keisuke ; Furugen, Ayako ; Narumi, Katsuya ; Ishikawa, Shuhei ; Saito, Yoshitaka ; Kobayashi, Masaki
著者名 Sakuma, Tomoya
著者名 Mukai, Yuto
著者名 Yamaguchi, Atsushi
著者名 Suganuma, Yudai
著者名 Okamoto, Keisuke
e-Rad_Researcher 90767261 著者名 Furugen, Ayako
e-Rad_Researcher 90746752 著者名 Narumi, Katsuya
e-Rad_Researcher 30880091 著者名 Ishikawa, Shuhei
e-Rad_Researcher 00835001 著者名 Saito, Yoshitaka
e-Rad_Researcher 70431319 著者名 Kobayashi, Masaki
言語	英語
キーワード	astrocytes-neuron lactate shuttle
	monocarboxylate transporter
	SH-SY5Y cell
	lactate
発行日	2024-04-04
出版者	The Pharmaceutical Society of Japan
出版者	日本薬学会
収録物名	Biological & pharmaceutical bulletin
巻(号)	47(4)
ページ	764-770
抄録	L-Lactate transport via monocarboxylate transporters (MCTs) in the central nervous system, represented by the astrocyte-neuron lactate shuttle (ANLS), is crucial for the maintenance of brain functions, including memory formation. Previously, we have reported that MCT1 contributes to L-lactate transport in normal human astrocytes. Therefore, in this study, we aimed to identify transporters that contribute to L-lactate transport in human neurons. SH-SY5Y cells, which are used as a model for human neurons, were differentiated using all-trans-retinoic acid. L-Lactate uptake was measured using radiolabeled L-lactate, and the expression of MCT proteins was confirmed Western blotting. L-Lactate transport was pH-dependent and saturated at high concentrations. Kinetic analysis suggested that L-lactate uptake was biphasic. Furthermore, MCT1, 2 selective inhibitors inhibited L-lactate transport. In addition, the expression of MCT1 and 2 proteins, but not MCT4, was confirmed. In this study, we demonstrated that MCT1 and 2 are major contributors to L-lactate transport in differentiated human neuroblastoma SH-SY5Y cells from the viewpoint of kinetic analysis. These results lead to a better understanding of ANLS in humans, and further exploration of the factors that can promote MCT1 and 2 functions is required.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0918-6158
EISSN	1347-5215
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1248/bpb.b23-00860