open access

https://hdl.handle.net/2115/92928

Peptide epimerase-dehydratase complex responsible for biosynthesis of the linaridin class ribosomal peptides

英語

peptide

2023-11

Oxford University Press

Bioscience biotechnology and biochemistry

87(11)

1316-1322

Grisemycin, salinipeptin, and cypemycin belong to the linaridin class of ribosomally synthesized and posttranslationally modified peptides that contain multiple dehydrobutyrine and D-amino acid residues. The biosynthetic gene clusters of these linaridins lack obvious candidate genes for the dehydratase and epimerase required to introduce dehydrobutyrine and D-amino acid residues, respectively. However, we previously demonstrated that the grisemycin (grm) cluster contained cryptic dehydratase and epimerase genes by heterologous expression of this biosynthetic gene cluster in Streptomyces lividans and proposed that two genes (grmH and grmL) with unknown functions catalyze dehydration and epimerization reactions. In this study, we confirmed that both GrmH and GrmL, which were shown to constitute a protein complex by a co-purification experiment, were required to catalyze the dehydration, epimerization, and proteolytic cleavage of a precursor peptide GrmA by in vivo experiments.

学術雑誌論文

Accepted Manuscript

This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience biotechnology and biochemistry following peer review. The version of record Wanlu Xiao, Takeshi Tsunoda, Chitose Maruyama, Yoshimitsu Hamano, Yasushi Ogasawara, Tohru Dairi, Peptide epimerase-dehydratase complex responsible for biosynthesis of the linaridin class ribosomal peptides, Bioscience, Biotechnology, and Biochemistry, Volume 87, Issue 11, November 2023, Pages 1316–1322 is available online at: https://doi.org/10.1093/bbb/zbad106.

0916-8451