Cryopreservation of masu salmon sperm using glucose-methanol extender and seminal plasma biomarkers related to post-thaw sperm motility

Fujimoto, Takafumi; Kaneyasu, Takahisa; Endoh, Mitsuru; Kogame, Yuya; Nynca, Joanna; Ciereszko, Andrzej; Takahashi, Eisuke; Yamaha, Etsuro; Naruse, Kiyoshi; Arai, Katsutoshi

Cryopreservation of masu salmon sperm using glucose-methanol extender and seminal plasma biomarkers related to post-thaw sperm motility

Fujimoto, Takafumi; Kaneyasu, Takahisa; Endoh, Mitsuru et al.

Aquaculture, 2022, 557, 738305

Permalink : https://hdl.handle.net/2115/93210

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アクセス権	open access
URI	https://hdl.handle.net/2115/93210
タイトル	Cryopreservation of masu salmon sperm using glucose-methanol extender and seminal plasma biomarkers related to post-thaw sperm motility
著者	Fujimoto, Takafumi ; Kaneyasu, Takahisa ; Endoh, Mitsuru ; Kogame, Yuya ; Nynca, Joanna ; Ciereszko, Andrzej ; Takahashi, Eisuke ; Yamaha, Etsuro ; Naruse, Kiyoshi ; Arai, Katsutoshi
e-Rad_Researcher 10400003 著者名 Fujimoto, Takafumi
著者名 Kaneyasu, Takahisa
著者名 Endoh, Mitsuru
著者名 Kogame, Yuya
著者名 Nynca, Joanna
著者名 Ciereszko, Andrzej
著者名 Takahashi, Eisuke
e-Rad_Researcher 60191376 著者名 Yamaha, Etsuro
e-Rad_Researcher 50208089 著者名 Naruse, Kiyoshi
e-Rad_Researcher 00137902 著者名 Arai, Katsutoshi
言語	英語
キーワード	Salmonids
	Spermatozoa
	Sperm motility
	Genebanking
発行日	2022
出版者	Elsevier
収録物名	Aquaculture
巻(号)	557
ページ	738305
抄録	The aim of this study was to evaluate the cryopreservation of masu salmon (Oncorhynchus masou) semen, using 0.18 M glucose in 9% methanol as an extender, and to characterize semen parameters (sperm motility; sperm concentration; and seminal plasma parameters such as pH, electric conductivity, DNA concentration, and protein concentration), with respect to post-thaw sperm motility. Semen samples were collected from fishes in the middle of the spawning season, at Toya Lake station, Hokkaido University, and the end of the spawning season, at the Nanae freshwater station, Hokkaido University. The semen was diluted with the extender at a 1:5 ratio (semen:extender) and then equilibrated for 15 min. The final concentration was 0.15 M and 7.5% for glucose and methanol, respectively. Sperm motility was assessed for fresh, fresh-diluted, and post-thaw semen using computer-assisted sperm analysis. The post-thaw sperm showed lower motility, compared with those of fresh and fresh-diluted sperm, which ranged from 33.9% to 82.9% and 1.5% to 27.1% in the semen collected in the middle and end the spawning season, respectively. Sperm concentration, pH, and electric conductivity of the semen collected at the end of the spawning season were lower than those of semen collected in the middle of the spawning season. Moreover, remarkably high DNA concentrations were detected in the semen at the end of the spawning season, although no difference was observed in protein concentration. Finally, semen cryopreserved using the aforementioned method could be used for artificial fertilization to produce offspring. These results indicate that the glucose-methanol extender is useful for sperm cryopreservation in masu salmon and that the suitability of semen for cryopreservation is affected by the condition of the parental fish.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0044-8486
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.aquaculture.2022.738305