Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length

Nakahara, Masaki; Takagi, Mutsumi; Hattori, Takako; Wakitani, Shigeyuki; Yoshida, Toshiomi

Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length

Nakahara, Masaki; Takagi, Mutsumi; Hattori, Takako et al.

Cytotechnology, 2005, 47(1-3), 19-27

Permalink : https://hdl.handle.net/2115/933

このアイテムのアクセス数:2,090件（2026-04-16 07:46 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
CT47(1-3)	pdf	131 KB	1,730

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/933
タイトル	Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length
著者	Nakahara, Masaki ; Takagi, Mutsumi ; Hattori, Takako ; Wakitani, Shigeyuki ; Yoshida, Toshiomi
著者名 Nakahara, Masaki
e-Rad_Researcher 20263212 著者名 Takagi, Mutsumi 高木, 睦
著者名 Hattori, Takako
著者名 Wakitani, Shigeyuki
著者名 Yoshida, Toshiomi
言語	英語
キーワード	Chondrogenesis
	Hematopoiesis
	Mesenchymal stem cell
	Population doubling level
	Subcultivation
	Telomere
発行日	2005-01
出版者	Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
収録物名	Cytotechnology
巻(号)	47(1-3)
ページ	19-27
抄録	Effects of subcultivation of human bone marrow mesenchymal stem cells on their capacities for chondrogenesis and supporting hematopoiesis, and telomea length were investigated. Mesenchymal stem cells were isolated from human bone marrow aspirates and subcultivated several times at 37℃ under a 5% CO2 atmosphere employing DMEM medium containing 10% FCS up to the 20th population doubling level (PDL). The ratio of CD45- CD105+ cells among these cells slightly increased as PDL increased. However, there was no marked change in the chondrogenic capacity of these cells, which was confirmed by expression assay of aggrecan mRNA and Safranin O staining after pellet cell cultivation. The change in capacity to support hematopoiesis of cord blood cells was not observed among cells with various PDLs. On the other hand, telomere length markedly decreased as PDL increased at a higher rate than that at which telomere length of primary mesenchymal stem cells decreased as the age of donor increased.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0920-9069
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10616-005-3752-9