Characterization of a mammalian orthoreovirus isolated from the large flying fox, Pteropus vampyrus, in Indonesia

Intaruck, Kittiya; Tabata, Koshiro; Itakura, Yukari; Kawaguchi, Nijiho; Kishimoto, Mai; Setiyono, Agus; Handharyani, Ekowati; Harima, Hayato; Kimura, Takashi; Hall, William W.; Orba, Yasuko; Sawa, Hirofumi; Sasaki, Michihito

Characterization of a mammalian orthoreovirus isolated from the large flying fox, Pteropus vampyrus, in Indonesia

Intaruck, Kittiya; Tabata, Koshiro; Itakura, Yukari et al.

Journal of General Virology, 2024, 105(9), 002028

Permalink : https://hdl.handle.net/2115/93301

このアイテムのアクセス数:292件（2026-10-06 07:12 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/93301
タイトル	Characterization of a mammalian orthoreovirus isolated from the large flying fox, Pteropus vampyrus, in Indonesia
著者	Intaruck, Kittiya ; Tabata, Koshiro ; Itakura, Yukari ; Kawaguchi, Nijiho ; Kishimoto, Mai ; Setiyono, Agus ; Handharyani, Ekowati ; Harima, Hayato ; Kimura, Takashi ; Hall, William W. ; Orba, Yasuko ; Sawa, Hirofumi ; Sasaki, Michihito
著者名 Intaruck, Kittiya
e-Rad_Researcher 70980837 著者名 Tabata, Koshiro
e-Rad_Researcher 00980856 著者名 Itakura, Yukari
著者名 Kawaguchi, Nijiho
e-Rad_Researcher 20822068 著者名 Kishimoto, Mai
著者名 Setiyono, Agus
著者名 Handharyani, Ekowati
e-Rad_Researcher 70805407 著者名 Harima, Hayato
e-Rad_Researcher 90261338 著者名 Kimura, Takashi
著者名 Hall, William W.
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
言語	英語
キーワード	Fruit bats
	Indonesia
	Mammalian orthoreovirus
	Viral pathogenicity
	Virus isolation
発行日	2024-09-25
出版者	Microbiology Society
収録物名	Journal of General Virology
巻(号)	105(9)
ページ	002028
抄録	Fruit bats serve as an important reservoir for many zoonotic pathogens, including Nipah virus, Hendra virus, Marburg virus and Lyssavirus. To gain a deeper insight into the virological characteristics, pathogenicity and zoonotic potential of bat-borne viruses, recovery of infectious viruses from field samples is important. Here, we report the isolation and characterization of a mammalian orthoreovirus (MRV) from a large flying fox (Pteropus vampyrus) in Indonesia, which is the first detection of MRV in Southeast Asia. MRV was recovered from faecal samples of three different P. vampyrus in Central Java. Nucleotide sequence analysis revealed that the genome of the three MRV isolates shared more than 99% nucleotide sequence identity. We tentatively named one isolated strain as MRV12-52 for further analysis and characterization. Among 10 genome segments, MRV12-52 S1 and S4, which encode the cell-attachment protein and outer capsid protein, had 93.6 and 95.1% nucleotide sequence identities with known MRV strains, respectively. Meanwhile, the remaining genome segments of MRV12-52 were divergent with 72.9–80.7 % nucleotide sequence identities. Based on the nucleotide sequence of the S1 segment, MRV12-52 was grouped into serotype 2, and phylogenetic analysis demonstrated evidence of past reassortment events. In vitro characterization of MRV12-52 showed that the virus efficiently replicated in BHK-21, HEK293T and A549 cells. In addition, experimental infection of laboratory mice with MRV12-52 caused severe pneumonia with 75% mortality. This study highlights the presence of pathogenic MRV in Indonesia, which could serve as a potential animal and public health concern.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution 4.0 International
権利情報	© Kittiya Intaruck, Koshiro Tabata, Yukari Itakura, Nijiho Kawaguchi, Mai Kishimoto, Agus Setiyono, Ekowati Handharyani, Hayato Harima, Takashi Kimura, William W. Hall, Yasuko Orba, Hirofumi Sawa and Michihito Sasaki, 2024. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, 105, 9, 2024, 10.1099/jgv.0.002028.
PISSN	0022-1317
EISSN	1465-2099
NCID	AA00698722
関連情報	PMID 39319430
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1099/jgv.0.002028