Evaluation of antifungal activities of woody plant barks using an incubation system with Trametes versicolor

Koyama, Masato; Tamai, Yutaka; Shigetomi, Kengo; Sano, Yuzou

Evaluation of antifungal activities of woody plant barks using an incubation system with Trametes versicolor

Koyama, Masato; Tamai, Yutaka; Shigetomi, Kengo et al.

Trees - Structure and Function, 2023, 38, 37-47

Permalink : https://hdl.handle.net/2115/93376

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/93376
タイトル	Evaluation of antifungal activities of woody plant barks using an incubation system with Trametes versicolor
著者	Koyama, Masato ; Tamai, Yutaka ; Shigetomi, Kengo ; Sano, Yuzou
著者名 Koyama, Masato
e-Rad_Researcher 50281796 著者名 Tamai, Yutaka
e-Rad_Researcher 20547202 著者名 Shigetomi, Kengo
e-Rad_Researcher 90226043 著者名 Sano, Yuzou
言語	英語
キーワード	Antifungal substances
	Bark function
	Constitutive chemical defense
	Gamma sterilization
	Sorbus commixta
	White-rot fungus
発行日	2023-11-06
出版者	Springer
収録物名	Trees - Structure and Function
巻(号)	38
ページ	37-47
抄録	Tree barks protect inside of tree bodies against pathogen invasion, but their chemical constitutive defense mechanisms remain largely unexplored. Therefore, the antifungal activities of bark tissues of 25 tree species acquired from Hokkaido, Japan, were examined to investigate the constitutive chemical defense mechanisms of tree barks. Bark pieces were placed on potato dextrose agar with Trametes versicolor after sterilization with gamma rays at 15 kGy. Bark tissues of most species exhibited limited activities. However, the whole and inner barks of Sorbus commixta completely inhibited the mycelial growth of T. versicolor. Furthermore, the inner bark of S. commixta on media with grooves also inhibited the mycelial growth of T. versicolor, indicating that antifungal substances generated from the inner bark of S. commixta are volatile. Among other species, Cerasus sargentii var. sargentii, Picea jezoensis var. jesoensis, and Magnolia obovate showed lower antifungal activities than S. commixta. We also examined the validity of 15 kGy for sterilizing the bark pieces of 16 species among 25 species. A dose of 12 kGy was sufficient to sterilize the bark pieces of all species, except Abies sachalinensis, and 15 kGy was necessary for the bark pieces of A. sachalinensis. In conclusion, this incubation system can be applied to select tree species, such as S. commixta, with high antifungal activities, and 15 kGy was sufficient for this incubation system to sterilize the barks of tree species in Hokkaido. This incubation system is useful to find out tree species that form bark tissues with unique chemical defense mechanisms.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00468-023-02464-8
PISSN	0931-1890
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00468-023-02464-8