Insight into the genetic diversity of Mycobacterium bovis isolated from cattle in Malawi

Kapalamula, Thoko Flav; Chizimu, Joseph Yamweka; Akapelwa, Mwangala Lonah; Barnes, David Atomanyi; Toyting, Jirachaya; Bwalya, Precious; Basikolo, Linda; Squarre, David; Chambaro, Herman M.; Gordon, Stephen V.; Thapa, Jeewan; Nakajima, Chie; Suzuki, Yasuhiko

Insight into the genetic diversity of Mycobacterium bovis isolated from cattle in Malawi

Kapalamula, Thoko Flav; Chizimu, Joseph Yamweka; Akapelwa, Mwangala Lonah et al.

Research in veterinary science, 2023, 164

Permalink : https://hdl.handle.net/2115/93677

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アクセス権	open access
URI	https://hdl.handle.net/2115/93677
タイトル	Insight into the genetic diversity of Mycobacterium bovis isolated from cattle in Malawi
著者	Kapalamula, Thoko Flav ; Chizimu, Joseph Yamweka ; Akapelwa, Mwangala Lonah ; Barnes, David Atomanyi ; Toyting, Jirachaya ; Bwalya, Precious ; Basikolo, Linda ; Squarre, David ; Chambaro, Herman M. ; Gordon, Stephen V. ; Thapa, Jeewan ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Kapalamula, Thoko Flav
著者名 Chizimu, Joseph Yamweka
著者名 Akapelwa, Mwangala Lonah
著者名 Barnes, David Atomanyi
著者名 Toyting, Jirachaya
著者名 Bwalya, Precious
著者名 Basikolo, Linda
著者名 Squarre, David
著者名 Chambaro, Herman M.
著者名 Gordon, Stephen V.
著者名 Thapa, Jeewan
著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Mycobacterium bovis
	Bovine tuberculosis
	Genetic diversity
	Deletion analysis
	Spoligotyping
	MIRU-VNTR
発行日	2023-11
出版者	Elsevier
収録物名	Research in veterinary science
巻(号)	164
抄録	We describe the genetic diversity and phylogenetic relationships of Mycobacterium bovis, isolated from cattle in Malawi. Deletion analysis, spoligotyping, and MIRU-VNTR typing were used to genotype the isolates. Combined with a larger dataset from neighboring countries, the overall M. bovis diversity in Southern Africa was contextualized. From the southern and northern regions of Malawi, 24 isolates were confirmed as M. bovis. We pooled data for the central region (60 isolates) from our recent publication to conceptualize the genetic and phylogenetic relationships of M. bovis in Malawi. European 1 was the dominant M. bovis clonal complex, with 10 unique spoligotype patterns, and SB0131 was ubiquitous. High genetic diversity, a low clustering rate, and many singletons, coupled with a low mutation transmission index, infer a low level of recent transmission, and suggest an endemic status of bovine tuberculosis (bTB) in Malawi. M. bovis isolates from Zambia, Mozambique, and South Africa were genetically related to Malawian isolates, whereas Tanzanian isolates were distantly related. The diversity and phylogenetic analysis suggest earlier introductions and maintenance of M. bovis by constant reinfection from reservoir animals. These findings are fundamental to understanding the source and route of infection in order to establish alternative management strategies for bTB.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0034-5288
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.rvsc.2023.105030