4.5SH RNA counteracts deleterious exonization of SINE B1 in mice

Yoshimoto, Rei; Nakayama, Yuta; Nomura, Ikuko; Yamamoto, Ikuko; Nakagawa, Yumeka; Tanaka, Shigeyuki; Kurihara, Misuzu; Suzuki, Yu; Kobayashi, Takehiko; Kozuka-Hata, Hiroko; Oyama, Masaaki; Mito, Mari; Iwasaki, Shintaro; Yamazaki, Tomohiro; Hirose, Tetsuro; Araki, Kimi; Nakagawa, Shinichi

4.5SH RNA counteracts deleterious exonization of SINE B1 in mice

Yoshimoto, Rei; Nakayama, Yuta; Nomura, Ikuko et al.

Molecular cell, 2023, 83(24), 4479-4493

Permalink : https://hdl.handle.net/2115/94052

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アクセス権	open access
URI	https://hdl.handle.net/2115/94052
タイトル	4.5SH RNA counteracts deleterious exonization of SINE B1 in mice
著者	Yoshimoto, Rei ; Nakayama, Yuta ; Nomura, Ikuko ; Yamamoto, Ikuko ; Nakagawa, Yumeka ; Tanaka, Shigeyuki ; Kurihara, Misuzu ; Suzuki, Yu ; Kobayashi, Takehiko ; Kozuka-Hata, Hiroko ; Oyama, Masaaki ; Mito, Mari ; Iwasaki, Shintaro ; Yamazaki, Tomohiro ; Hirose, Tetsuro ; Araki, Kimi ; Nakagawa, Shinichi
著者名 Yoshimoto, Rei
著者名 Nakayama, Yuta
著者名 Nomura, Ikuko
著者名 Yamamoto, Ikuko
著者名 Nakagawa, Yumeka
著者名 Tanaka, Shigeyuki
著者名 Kurihara, Misuzu
著者名 Suzuki, Yu
著者名 Kobayashi, Takehiko
著者名 Kozuka-Hata, Hiroko
著者名 Oyama, Masaaki
著者名 Mito, Mari
著者名 Iwasaki, Shintaro
e-Rad_Researcher 90732280 著者名 Yamazaki, Tomohiro
e-Rad_Researcher 30273220 著者名 Hirose, Tetsuro
著者名 Araki, Kimi
e-Rad_Researcher 50324679 著者名 Nakagawa, Shinichi
言語	英語
キーワード	4.5SH RNA
	noncoding RNA
	exon skipping
	alternative splicing
	retrotransposon
	SINE B1
	Hnrnpm
発行日	2023-12-21
出版者	Cell Press
収録物名	Molecular cell
巻(号)	83(24)
ページ	4479-4493
抄録	4.5SH RNA is a highly abundant, small rodent-specific noncoding RNA that localizes to nuclear speckles en-riched in pre-mRNA-splicing regulators. To investigate the physiological functions of 4.5SH RNA, we have created mutant mice that lack the expression of 4.5SH RNA. The mutant mice exhibited embryonic lethality, suggesting that 4.5SH RNA is an essential species-specific noncoding RNA in mice. RNA-sequencing ana-lyses revealed that 4.5SH RNA protects the transcriptome from abnormal exonizations of the antisense inser-tions of the retrotransposon SINE B1 (asB1), which would otherwise introduce deleterious premature stop codons or frameshift mutations. Mechanistically, 4.5SH RNA base pairs with complementary asB1-contain-ing exons via the target recognition region and recruits effector proteins including Hnrnpm via its 50 stem loop region. The modular organization of 4.5SH RNA allows us to engineer a programmable splicing regulator to induce the skipping of target exons of interest. Our results also suggest the general existence of splicing reg-ulatory noncoding RNAs.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1097-2765
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.molcel.2023.11.019