Experimental demonstration of the transmission of Spiroplasma between different arthropod taxa

Ogata, Shohei; Hayashi, Naoki; Eshita, Yuki; Nagasawa, Yasuha; Nonaka, Nariaki; Nakao, Ryo

Experimental demonstration of the transmission of Spiroplasma between different arthropod taxa

Ogata, Shohei; Hayashi, Naoki; Eshita, Yuki et al.

Journal of Medical Entomology, 2024, 61(3), 733-740

Permalink : https://hdl.handle.net/2115/94102

このアイテムのアクセス数:138件（2026-08-07 21:12 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Ogata_JME_v3_Clean	pdf	851 KB	124

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/94102
タイトル	Experimental demonstration of the transmission of Spiroplasma between different arthropod taxa
著者	Ogata, Shohei ; Hayashi, Naoki ; Eshita, Yuki ; Nagasawa, Yasuha ; Nonaka, Nariaki ; Nakao, Ryo
著者名 Ogata, Shohei
著者名 Hayashi, Naoki
著者名 Eshita, Yuki
著者名 Nagasawa, Yasuha
e-Rad_Researcher 50281853 著者名 Nonaka, Nariaki
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
言語	英語
キーワード	Spiroplasma
	horizontal transmission
	mosquito
	symbiont
	tick
発行日	2024-02-21
出版者	Oxford University Press
収録物名	Journal of Medical Entomology
巻(号)	61(3)
ページ	733-740
抄録	Spiroplasma (Mycoplasmatales: Spiroplasmataceae) is one of the most widely distributed symbionts of arthropods. Spiroplasma species can infect their hosts via vertical or horizontal transmission. However, the mode of transmission of Spiroplasma between different arthropod taxa has not been elucidated. In this study, we investigated the potential for the transmission of Spiroplasma to non-native arthropod species, using 2 Spiroplasma spp. isolated from ticks, namely Spiroplasma ixodetis and Spiroplasma mirum, and 3 species of mosquito laboratory colonies, namely Aedes albopictus, Aedes aegypti, and Culex pipiens pallens (Diptera: Culicidae). After feeding the adult mosquitoes with Spiroplasma-containing artificial meals, they were kept at 25 degrees C for 10 days. Homogenates prepared from Spiroplasma-fed mosquitoes were used to re-isolate Spiroplasma using the in vitro culture method. Nine weeks after culture initiation, the presence of Spiroplasma was tested using the polymerase chain reaction (PCR). The results revealed that only S. ixodetis was detected from all 3 species of mosquitoes and re-isolated from 2 of them. The differences in the infection ability of different Spirolasma species could be attributed to several factors, including environmental effects. Nevertheless, this is the first experimental demonstration of Spiroplasma transmission among different arthropod taxa. Further studies are needed to elucidate the evolutionary mechanism that supports the survival of Spiroplasma in nature. Graphical Abstract
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Medical Entomology following peer review. The version of record Shohei Ogata, Naoki Hayashi, Yuki Eshita, Yasuha Nagasawa, Nariaki Nonaka, Ryo Nakao, Experimental demonstration of the transmission of Spiroplasma between different arthropod taxa, Journal of Medical Entomology, Volume 61, Issue 3, May 2024, Pages 733–740, is available online at: https://doi.org/10.1093/jme/tjae020
PISSN	0022-2585
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/jme/tjae020