Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor

Pal, Aniruddha; Oyane, Ayako; Nakamura, Maki; Koga, Kenji; Nishida, Erika; Miyaji, Hirofumi

Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor

Pal, Aniruddha; Oyane, Ayako; Nakamura, Maki et al.

International Journal of Molecular Sciences, 2024, 25(3), 1495

Permalink : https://hdl.handle.net/2115/94718

このアイテムのアクセス数:150件（2026-07-17 19:52 集計）

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ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.3390/ijms25031495			42

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/94718
タイトル	Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor
著者	Pal, Aniruddha ; Oyane, Ayako ; Nakamura, Maki ; Koga, Kenji ; Nishida, Erika ; Miyaji, Hirofumi
著者名 Pal, Aniruddha
e-Rad_Researcher 50356672 著者名 Oyane, Ayako
e-Rad_Researcher 00568925 著者名 Nakamura, Maki
e-Rad_Researcher 30356969 著者名 Koga, Kenji
e-Rad_Researcher 50779882 著者名 Nishida, Erika
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
言語	英語
キーワード	apatite
	collagen
	basic fibroblast growth factor (bFGF)
	tailored release
	scaffolds
	fluoride
発行日	2024-01-25
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	25(3)
ページ	1495
抄録	Coating layers consisting of a crystalline apatite matrix with immobilized basic fibroblast growth factor (bFGF) can release bFGF, thereby enhancing bone regeneration depending on their bFGF content. We hypothesized that the incorporation of fluoride ions into apatite crystals would enable the tailored release of bFGF from the coating layer depending on the layer’s fluoride content. In the present study, coating layers consisting of fluoride-incorporated apatite (FAp) crystals with immobilized bFGF were coated on a porous collagen sponge by a precursor-assisted biomimetic process using supersaturated calcium phosphate solutions with various fluoride concentrations. The fluoride content in the coating layer increased with the increasing fluoride concentration of the supersaturated solution. The increased fluoride content in the coating layer reduced its solubility and suppressed the burst release of bFGF from the coated sponge into a physiological salt solution. The bFGF release was caused by the partial dissolution of the coating layer and, thus, accompanied by the fluoride release. The concentrations of released bFGF and fluoride were controlled within the estimated effective ranges in enhancing bone regeneration. These findings provide useful design guidelines for the construction of a mineralized, bFGF-releasing collagen scaffold that would be beneficial for bone tissue engineering, although further in vitro and in vivo studies are warranted.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1422-0067
関連情報	PMID 38338772
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms25031495