Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation

Nakano, Shintaro; Kasai, Machiko; Nakamura, Keisuke; Akasaka, Tsukasa; Yoshida, Yasuhiro; Suzuki, Shiho; Ohiro, Yoichi; Hasebe, Akira

Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation

Nakano, Shintaro; Kasai, Machiko; Nakamura, Keisuke et al.

Journal of oral biosciences, 2024, 66(3), 594-604

Permalink : https://hdl.handle.net/2115/95064

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アクセス権	open access
URI	https://hdl.handle.net/2115/95064
タイトル	Epithelial-mesenchymal transition in oral cancer cells induced by prolonged and persistent Fusobacterium nucleatum stimulation
著者	Nakano, Shintaro ; Kasai, Machiko ; Nakamura, Keisuke ; Akasaka, Tsukasa ; Yoshida, Yasuhiro ; Suzuki, Shiho ; Ohiro, Yoichi ; Hasebe, Akira
著者名 Nakano, Shintaro
著者名 Kasai, Machiko
著者名 Nakamura, Keisuke
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
e-Rad_Researcher 90281162 著者名 Yoshida, Yasuhiro
著者名 Suzuki, Shiho
e-Rad_Researcher 40301915 著者名 Ohiro, Yoichi
e-Rad_Researcher 90281815 著者名 Hasebe, Akira
言語	英語
キーワード	Oral cancer
	Fusobacterium nucleatum
	Epithelial-mesenchymal transition (EMT)
	Dexamethasone
発行日	2024-09
出版者	Elsevier
収録物名	Journal of oral biosciences
巻(号)	66(3)
ページ	594-604
抄録	Objectives: Several studies have reported the effects of Fusobacterium nucleatum stimulation on oral cancer cells. However, given that these studies typically span a stimulation period of three days to eight days, the in vitro studies conducted to date may not fully mimic the oral cancer environment, which involves constant exposure to oral commensal bacteria. This study aimed to elucidate the effects of prolonged and persistent Fusobacterium nucleatum infection on oral cancer cells. Methods: Human tongue squamous cell carcinoma (SCC) cells were continuously stimulated with Fusobacterium nucleatum for two or four weeks, then experimentally evaluated. Results: Prolonged, persistent Fusobacterium nucleatum stimulation increased the cells' proliferative, invasive, and migratory capacities, decreased their expression of epithelial markers, and increased their expression of mesenchymal markers progressively with time. The cells also adopted a spindle-shaped morphology and cell-tocell contact dependence was progressively lost, suggesting time-dependent occurrence of epithelial-mesenchymal transition. Furthermore, mRNA levels of CD44, a cancer stem cell marker, were time-dependently upregulated. When SCC cells were stimulated with Fusobacterium nucleatum for four weeks in the presence of dexamethasone, Fusobacterium nucleatum induced epithelial-mesenchymal transition was inhibited. Conclusions: Epithelial-mesenchymal transition in human tongue SCC cells was time-dependently induced by prolonged, persistent Fusobacterium nucleatum stimulation and inhibited by dexamethasone. Routine decontamination of the oral cavity may be crucial for controlling tumor invasion and metastasis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1349-0079
関連情報	PMID 38782256
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.job.2024.05.006