Regional difference in the distribution of alkaline phosphatase, PHOSPHO1, and calcein labeling in the femoral metaphyseal trabeculae in parathyroid hormone-administered mice

Haraguchi-Kitakamae, Mai; Nakajima, Yuhi; Yamamoto, Tomomaya; Hongo, Hiromi; Cui, Jiaxin; Shi, Yan; Liu, Xuanyu; Yao, Qi; Maruoka, Haruhi; Abe, Miki; Sekiguchi, Tamaki; Yokoyama, Ayako; Amizuka, Norio; Sasano, Yasuyuki; Hasegawa, Tomoka

Regional difference in the distribution of alkaline phosphatase, PHOSPHO1, and calcein labeling in the femoral metaphyseal trabeculae in parathyroid hormone-administered mice

Haraguchi-Kitakamae, Mai; Nakajima, Yuhi; Yamamoto, Tomomaya et al.

Journal of oral biosciences, 2024, 66(3), 554-566

Permalink : https://hdl.handle.net/2115/95578

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アクセス権	open access
URI	https://hdl.handle.net/2115/95578
タイトル	Regional difference in the distribution of alkaline phosphatase, PHOSPHO1, and calcein labeling in the femoral metaphyseal trabeculae in parathyroid hormone-administered mice
著者	Haraguchi-Kitakamae, Mai ; Nakajima, Yuhi ; Yamamoto, Tomomaya ; Hongo, Hiromi ; Cui, Jiaxin ; Shi, Yan ; Liu, Xuanyu ; Yao, Qi ; Maruoka, Haruhi ; Abe, Miki ; Sekiguchi, Tamaki ; Yokoyama, Ayako ; Amizuka, Norio ; Sasano, Yasuyuki ; Hasegawa, Tomoka
著者名 Haraguchi-Kitakamae, Mai
著者名 Nakajima, Yuhi
e-Rad_Researcher 50928299 著者名 Yamamoto, Tomomaya
e-Rad_Researcher 00778970 著者名 Hongo, Hiromi
著者名 Cui, Jiaxin
著者名 Shi, Yan
著者名 Liu, Xuanyu
著者名 Yao, Qi
e-Rad_Researcher 40962577 著者名 Maruoka, Haruhi
e-Rad_Researcher 40999603 著者名 Abe, Miki
e-Rad_Researcher 80580943 著者名 Sekiguchi, Tamaki
e-Rad_Researcher 20849111 著者名 Yokoyama, Ayako
e-Rad_Researcher 30242431 著者名 Amizuka, Norio
e-Rad_Researcher 30196191 著者名 Sasano, Yasuyuki
e-Rad_Researcher 50739349 著者名 Hasegawa, Tomoka
言語	英語
キーワード	Bone tissue
	Parathyroid hormone
	Alkaline phosphatase
	PHOSPHO1
	Immunohistochemistry
発行日	2024-09
出版者	Elsevier
収録物名	Journal of oral biosciences
巻(号)	66(3)
ページ	554-566
抄録	Objectives: This study aimed to elucidate whether the administration of parathyroid hormone (PTH) results in remodeling- or modeling-based bone formation in different regions of the murine femora, and whether the PTHdriven bone formation would facilitate osteoblastic differentiation into osteocytes. Methods: Six-week-old male C57BL/6J mice were employed to examine the distribution of alkaline phosphatase (ALP), PHOSPHO1, podoplanin, and calcein labeling in two distinct long bone regions: the metaphyseal trabeculae close to the chondro-osseous junction (COJ) and those distant from the COJ in three mouse groups, a control group receiving a vehicle (sham group) and groups receiving hPTH (1-34) twice a day (PTH BID group) or four times a day (PTH QID group) for two weeks. Results: The sham group showed PHOSPHO1-reactive mature osteoblasts localized primarily at the COJ, whereas the PTH BID/QID groups exhibited extended lines of PHOSPHO1-reactive osteoblasts even in regions distant from the COJ. The PTH QID group displayed fragmented calcein labeling in trabeculae close to the COJ, whereas continuous labeling was observed in trabeculae distant from the COJ. Osteoblasts tended to express podoplanin and PHOSPHO1 independently in the close and distant regions of the sham group, while osteoblasts in the PTHadministered groups showed immunoreactivity of podoplanin and PHOSPHO1 together in the close and distant regions. Conclusions: Administration of PTH may accelerate remodeling-based bone formation in regions close to the COJ while predominantly inducing modeling-based bone formation in distant regions. PTH appeared to simultaneously facilitate osteoblastic bone mineralization and differentiation into osteocytes in both remodeling- and modeling-based bone formation.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1349-0079
関連情報	PMID 38942193
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.job.2024.06.007