open access

https://hdl.handle.net/2115/95769

Cell cycle- and dose-dependent effects on mitochondrial DNA copy number variation following irradiation

英語

Radiation

2025-07-18

Company of Biologists

Journal of Cell Science

138(15)

263642

Cell survival after irradiation depends on the cell cycle at the time of exposure. This has been thought to be due to cell cycle-dependent nuclear DNA damage repair mechanisms. Here we show the relationships between the exposed dose, the cell cycle phase at the time of exposure, and changes in mitochondrial DNA copy numbers (mtDNAcn) after irradiation. We used a fluorescent ubiquitination-based cell cycle indicator (FUCCI), which allows visualization of the cell cycle, and confirmed cell cycle synchronization in human cervical HeLa cells. In synchronous HeLa-FUCCI cells, the mtDNAcn changed with the progression of the cell cycle. Also, G1 phase-synchronized cells showed a dose-dependent increase of mtDNAcn at 48 h after X-ray exposure, while G2 cells showed a dose-dependent increase at 24 h. In addition, S phase-synchronized cells showed a dose-dependent increase at 24 and 48 h after irradiation. These results showed the cell cycle- and dose-dependent effects on mtDNAcn after irradiation, which may shed light on the emerging role of mitochondrial genome and in cell survival.

学術雑誌論文

Version of Record

Creative Commons Attribution 4.0 International

0021-9533

1477-9137

AA11720335