Neutrophil extracellular trap formation and deoxyribonuclease I activity in patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis

Morita, Shinya; Nakamaru, Yuji; Fukuda, Atsushi; Fujiwara, Keishi; Suzuki, Masanobu; Hoshino, Kimiko; Honma, Aya; Nakazono, Akira; Homma, Akihiro

Neutrophil extracellular trap formation and deoxyribonuclease I activity in patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis

Morita, Shinya; Nakamaru, Yuji; Fukuda, Atsushi et al.

International archives of allergy and immunology, 2024, 185(8), 810-819

Permalink : https://hdl.handle.net/2115/95898

このアイテムのアクセス数:111件（2026-08-27 10:55 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/95898
タイトル	Neutrophil extracellular trap formation and deoxyribonuclease I activity in patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis
著者	Morita, Shinya ; Nakamaru, Yuji ; Fukuda, Atsushi ; Fujiwara, Keishi ; Suzuki, Masanobu ; Hoshino, Kimiko ; Honma, Aya ; Nakazono, Akira ; Homma, Akihiro
著者名 Morita, Shinya
e-Rad_Researcher 20344509 著者名 Nakamaru, Yuji
著者名 Fukuda, Atsushi
e-Rad_Researcher 70600146 著者名 Fujiwara, Keishi
e-Rad_Researcher 70455658 著者名 Suzuki, Masanobu
著者名 Hoshino, Kimiko
著者名 Honma, Aya
e-Rad_Researcher 90581041 著者名 Nakazono, Akira
e-Rad_Researcher 30312359 著者名 Homma, Akihiro
言語	英語
キーワード	Neutrophil extracellular traps
	Myeloperoxidase-deoxyribonucleic acid complex
	Citrullinated-histone H3-deoxyribonucleic acid complex
	Deoxyribonuclease I
	Otitis media with antineutrophil cytoplasmic antibody-associated vasculitis
発行日	2024-08-01
出版者	Karger
収録物名	International archives of allergy and immunology
巻(号)	185(8)
ページ	810-819
抄録	Introduction: No previous studies have evaluated the levels of neutrophil extracellular trap (NET) remnants or the importance of deoxyribonuclease (DNase) I activity based on the disease activity of otitis media with antineutrophil cytoplasmic antibody-associated vasculitis (OMAAV). The aim of this study was to explore the formation of NETs in the middle ear of patients with OMAAV during the onset and remission phases of the disease, with a particular focus on the relationships between the quantifiable levels of NET remnants and DNase I activity. Methods: OMAAV patients were eligible for inclusion. Patients with otitis media with effusion (OME) were examined as controls. The levels of cell-free deoxyribonucleic acid (DNA), citrullinated-histone H3 (cit-H3)-DNA complex, and myeloperoxidase (MPO)-DNA complex were quantified using an enzyme-linked immunosorbent assay. DNase I activity was measured using a fluorometric method. Results: The quantifiable levels of cell-free DNA, cit-H3-DNA complex, and MPO-DNA complex in the middle ear lavage of patients with OMAAV at onset were significantly higher than those in patients with OMAAV at remission and in patients with OME. DNase I activity in the patients with OMAAV at onset was significantly lower than those in patients with OMAAV at remission and OME and was negatively correlated with the level of MPO-DNA complex. Conclusions: This study suggests that NET remnants and DNase I activity may be potentially useful biomarkers for the diagnosis and disease activity of OMAAV.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer-reviewed but unedited manuscript version of the following article:Shinya Morita, Yuji Nakamaru, Atsushi Fukuda, Keishi Fujiwara, Masanobu Suzuki, Kimiko Hoshino, Aya Honma, Akira Nakazono, Akihiro Homma; Neutrophil Extracellular Trap Formation and Deoxyribonuclease I Activity in Patients with Otitis Media with Antineutrophil Cytoplasmic Antibody-Associated Vasculitis. Int Arch Allergy Immunol 7 August 2024; 185 (8): 810–819. https://doi.org/10.1159/000537927. The final, published version is available at http://karger.com/?doi=10.1159/000537927.
PISSN	1018-2438
EISSN	1423-0097
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1159/000537927