Application of bacterial-derived long cellulose nanofiber to suspension culture of mammalian cells as a shear protectant

Kaneko, Eiichiro; Tsujisaki, Haruto; Fujiwara, Masashi; Ando, Hidenori; Sato, Yasushi; Ishida, Tatsuhiro; Tani, Hirofumi; Tajima, Kenji

Application of bacterial-derived long cellulose nanofiber to suspension culture of mammalian cells as a shear protectant

Kaneko, Eiichiro; Tsujisaki, Haruto; Fujiwara, Masashi et al.

International Journal of Biological Macromolecules, 2024, 280(Part 3), 135938

Permalink : https://hdl.handle.net/2115/96005

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/96005
タイトル	Application of bacterial-derived long cellulose nanofiber to suspension culture of mammalian cells as a shear protectant
著者	Kaneko, Eiichiro ; Tsujisaki, Haruto ; Fujiwara, Masashi ; Ando, Hidenori ; Sato, Yasushi ; Ishida, Tatsuhiro ; Tani, Hirofumi ; Tajima, Kenji
著者名 Kaneko, Eiichiro
著者名 Tsujisaki, Haruto
e-Rad_Researcher 30229075 著者名 Fujiwara, Masashi
著者名 Ando, Hidenori
e-Rad_Researcher 30767778 著者名 Sato, Yasushi
e-Rad_Researcher 50325271 著者名 Ishida, Tatsuhiro
e-Rad_Researcher 10271644 著者名 Tani, Hirofumi
e-Rad_Researcher 00271643 著者名 Tajima, Kenji
言語	英語
キーワード	Nanofibrillated bacterial cellulose
	Mammalian cell culture
	Shear protectant
発行日	2024-11
出版者	Elsevier
収録物名	International Journal of Biological Macromolecules
巻(号)	280(Part 3)
ページ	135938
抄録	Nanofibrillated bacterial cellulose (NFBC) is a bio-compatible long-fiber nanocellulose produced by cellulosesynthesizing bacteria. It forms an entangled network structure in the suspension state, thereby imparting greater viscosity than conventional media additives. In this study, we examined its application as a shear protectant in the suspension culture of mammalian cells to mitigate hydrodynamic stress imposed on the cells. The media supplemented with hydroxypropyl cellulose-adsorbed NFBC (HP-NFBC) exhibited an increase in shear viscosity according to rheometric analysis, similar to FP003, a commercially available medium additive. Suspension culture of Chinese hamster ovary cells in HP-NFBC-containing media under a high stirring rate (120 rpm) demonstrated higher cell growth and lower cell death compared to those in the medium without additives and in FP003. A 0.10 (w/v)% concentration of HP-NFBC showed the highest viable cell number among the tested concentrations. Computational fluid dynamics simulation revealed a decrease in shear rate and flow velocity within the spinner flask owing to the addition of HP-NFBC or FP003. It is suggested that the decline of these parameters in high-viscosity media suppresses the hydrodynamic stress on cells. This study highlights the potential of HP-NFBC as a shear protectant in mammalian cell suspension culture.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© <2024>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0141-8130
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ijbiomac.2024.135938