Development of an Evaluation System Using Intestinal Organoids for Drug Efflux Transport Analysis by an Imaging Approach

Koseki, Chihiro; Ishikawa, Takehiko; Sato, Yuki; Shimada, Mikiko; Yokoi, Yuki; Nakamura, Kiminori; Honma, Naoyuki; Moriyama, Takanori; Kashiwagi, Hitoshi; Sugawara, Mitsuru

Development of an Evaluation System Using Intestinal Organoids for Drug Efflux Transport Analysis by an Imaging Approach

Koseki, Chihiro; Ishikawa, Takehiko; Sato, Yuki et al.

Journal of Pharmaceutical Sciences, 2024, 113(9), 2675-2682

Permalink : https://hdl.handle.net/2115/96131

このアイテムのアクセス数:146件（2026-10-09 04:22 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/96131
タイトル	Development of an Evaluation System Using Intestinal Organoids for Drug Efflux Transport Analysis by an Imaging Approach
著者	Koseki, Chihiro ; Ishikawa, Takehiko ; Sato, Yuki ; Shimada, Mikiko ; Yokoi, Yuki ; Nakamura, Kiminori ; Honma, Naoyuki ; Moriyama, Takanori ; Kashiwagi, Hitoshi ; Sugawara, Mitsuru
著者名 Koseki, Chihiro
著者名 Ishikawa, Takehiko
e-Rad_Researcher 00564981 著者名 Sato, Yuki
著者名 Shimada, Mikiko
e-Rad_Researcher 10895824 著者名 Yokoi, Yuki
e-Rad_Researcher 80381276 著者名 Nakamura, Kiminori
著者名 Honma, Naoyuki
e-Rad_Researcher 20312423 著者名 Moriyama, Takanori
e-Rad_Researcher 60510609 著者名 Kashiwagi, Hitoshi
e-Rad_Researcher 60332467 著者名 Sugawara, Mitsuru
言語	英語
キーワード	Enteroids
	Efflux transporter
	P-glycoprotein
	Passage
	Cryopreservation
発行日	2024-09
出版者	Elsevier
収録物名	Journal of Pharmaceutical Sciences
巻(号)	113(9)
ページ	2675-2682
抄録	There are several in vitro systems that enable evaluation of the absorption direction, but there are few quantitative systems that enable easy evaluation of the excretion direction. Enteroids, organoids derived from intestine, have been frozen and passaged for various research. But it is not clear how the freezing and passaging affect the expression and function of transporters. We investigated the effects of passage and cryopreservation of enteroids. We focused on P-gp (P-glycoprotein) and compared the transfer rates of rhodamine 123 (Rh123) into the lumen of enteroids with and without a P-gp inhibitor. mRNA expression levels did not change significantly before and after passage and cryopreservation. Accumulation of Rh123 in the lumen of enteroids was observed. With some P-gp inhibitors, excretion of Rh123 into the lumen of enteroids was inhibited and the nonexcreted Rh123 accumulated in enteroids epithelial cells. The transfer rate of Rh123 into the lumen of enteroids with a P-gp inhibitor was significantly decreased compared to that of without a P-gp inhibitor. Before and after passage and cryopreservation, the transfer rate was almost the same as that of primary cultured enteroids. We succeeded in easily evaluating whether a component is a substrate of P-gp using enteroids. (c) 2024 American Pharmacists Association. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0022-3549
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.xphs.2024.06.007