open access

https://hdl.handle.net/2115/96134

Induction of apoptotic cell death by calcitriol in RAW264.7 cells

英語

apoptosis

2025-09-15

Hokkaido association for dental science

Hokkaido journal of dental science

46

44-53

Vitamin D affects bone and mineral metabolism and immunity. Epidemiological data suggests that vitamin D may play a role in the development and progression of cancer. Additionally, calcitriol, an active form of vitamin D, has an antiproliferative effect on malignant tumo rs. However, it has also been reported that calcitriol suppresses cell death, suggesting that the effects of calcitriol may vary depending on the type of cell. In this study, we aimed to reveal the effects of calcitriol sti mulation on macrophage-like cells derived from tumors induced by murine leukemia virus (RAW264.7 cells) and the cell death pathway.　RAW264.7 cells were stimulated with calcitriol. Cell death was de termined by measuring the lactate dehydrogenase (LDH) levels in the supernatant and the percentage of cells stained with annexin V/propidium iodide (PI). Cell death pathways were examined using Western blotting.　LDH levels were increased in concentration- and time-dependent manners after calcitriol stimulation. Annexin V/PI staining showed that calcitriol stimulation increased the percentage of cells stained with both annexin V and PI compared with that of the control. Calcitriol stimulation increased the expression of cleaved caspase-3 in the supernatants. A necroptosis inhibitor, Necrostatin-1, did not affect the leve l of LDH induced by calcitriol stimulation.　Calcitriol induces cell death (apoptosis; not necroptosis) in RAW264.7 cells.

学術雑誌論文

Version of Record

0914-7063

AN00229778