Role of heavy water in modified university of wisconsin solution for extended cold storage of rat liver

Fukai, Moto; Shibata, Kengo; Sakamoto, Sodai; Ishikawa, Takahisa; Kawamura, Norio; Fujiyoshi, Masato; Fujiyoshi, Sunao; Nakamura, Kosei; Bochimoto, Hiroki; Shimada, Shingo; Shimamura, Tsuyoshi; Taketomi, Akinobu

Role of heavy water in modified university of wisconsin solution for extended cold storage of rat liver

Fukai, Moto; Shibata, Kengo; Sakamoto, Sodai et al.

Transplantation proceedings, 2024, 56(8), 1890-1895

Permalink : https://hdl.handle.net/2115/96170

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アクセス権	open access
URI	https://hdl.handle.net/2115/96170
タイトル	Role of heavy water in modified university of wisconsin solution for extended cold storage of rat liver
著者	Fukai, Moto ; Shibata, Kengo ; Sakamoto, Sodai ; Ishikawa, Takahisa ; Kawamura, Norio ; Fujiyoshi, Masato ; Fujiyoshi, Sunao ; Nakamura, Kosei ; Bochimoto, Hiroki ; Shimada, Shingo ; Shimamura, Tsuyoshi ; Taketomi, Akinobu
e-Rad_Researcher 60374344 著者名 Fukai, Moto
e-Rad_Researcher 90962853 著者名 Shibata, Kengo
著者名 Sakamoto, Sodai
著者名 Ishikawa, Takahisa
著者名 Kawamura, Norio
著者名 Fujiyoshi, Masato
著者名 Fujiyoshi, Sunao
著者名 Nakamura, Kosei
著者名 Bochimoto, Hiroki
著者名 Shimada, Shingo
著者名 Shimamura, Tsuyoshi
e-Rad_Researcher 70363364 著者名 Taketomi, Akinobu
言語	英語
キーワード	Liver
	Cold storage
	Preservation
	Isolated perfused rat liver
	IPRL
	Heavy water
	D2O
発行日	2024-10
出版者	Elsevier
収録物名	Transplantation proceedings
巻(号)	56(8)
ページ	1890-1895
抄録	To resolve the critical donor shortage worldwide, enlarging the potential donor pool to include expanded criteria donors is necessary. Despite numerous attempts to establish new preservation solutions, no dramatic innovation has occurred since University of Wisconsin (UW) solution displaced Euro Collins’ solution; UW solution remains the global gold standard. We previously developed a heavy water (D2O)–containing organ storage solution, Dsol, which is effective for livers subjected to extended cold storage (CS), and reported its effectiveness. Dsol is a modified UW solution; however, the substances or conditions that exhibit a synergistic or additive effect with D2O are unclear. Here we made UWD solution by removing hydroxyethyl starch (HES) from and adding 30%-D2O to UW solution, and compared the effects of these solutions. After 48 hours of CS, the livers were reperfused at 37 °C on an isolated perfused rat liver apparatus, and their perfusion kinetics, functions, and injuries were compared. In the UW group, portal vein resistance significantly increased and the oxygen consumption rate and bile production decreased; in contrast, these changes were suppressed in the UWD group. Organ expansion and liver damage progressed in both groups. These results confirmed that the removal of HES from and addition of D2O to the UW solution reduced CS-induced cellular function impairments and microcirculatory disorders. However, to reduce injury during reperfusion after CS, it is necessary to provide conditions that inhibit injury progression after reperfusion.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0041-1345
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.transproceed.2024.06.005